KGF 2 His Human

Keratinocyte Growth Factor-2 Human Recombinant, His Tag
Cat. No.
BT19298
Source
E.coli.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

KGF 2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (38-208) and having a molecular mass of 22.0kDa.
KGF 2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Keratinocyte growth factor 2 (KGF-2), also known as fibroblast growth factor 10 (FGF-10), is a member of the fibroblast growth factor (FGF) family. FGFs are involved in various biological processes, such as embryonic development, cell growth, tissue repair, and tumor growth. KGF-2 specifically stimulates the growth and differentiation of epithelial cells, particularly keratinocytes in the skin. It plays a crucial role in wound healing, hair follicle development, and lung morphogenesis.
Description
Recombinant human KGF-2 is produced in E. coli and consists of a single polypeptide chain of 196 amino acids (residues 38-208). It has a molecular weight of 22.0 kDa. This non-glycosylated protein is fused to a 25-amino acid His-tag at the N-terminus to facilitate purification by chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The KGF-2 solution is provided at a concentration of 0.25 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 2 mM DTT, 2 mM EDTA, and 50% glycerol.
Stability
For short-term storage (up to 4 weeks), store the KGF-2 solution at 4°C. For long-term storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of KGF-2 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMQALGQ DMVSPEATNS SSSSFSSPSS AGRHVRSYNH LQGDVRWRKL FSFTKYFLKI EKNGKVSGTK KENCPYSILE ITSVEIGVVA VKAINSNYYL AMNKKGKLYG SKEFNNDCKL KERIEENGYN TYASFNWQHN GRQMYVALNG KGAPRRGQKT RRKNTSAHFL PMVVHS

Product Science Overview

Introduction

Keratinocyte Growth Factor-2 (KGF-2), also known as Fibroblast Growth Factor 10 (FGF-10), is a member of the fibroblast growth factor family. This family includes at least 23 related peptides that play crucial roles in various biological processes, including cell growth, development, and tissue repair .

Structure and Characteristics

KGF-2 is a single, non-glycosylated polypeptide chain containing 170 amino acids, with a molecular mass of approximately 19,300 Daltons . It shares 57% sequence homology with KGF-1 (FGF-7) and binds to the same receptor . The human recombinant version of KGF-2 is often produced in E. coli and tagged with a His (histidine) tag to facilitate purification and detection .

Biological Function

KGF-2 is known for its role in stimulating the proliferation and migration of epithelial cells, particularly keratinocytes. It is involved in various physiological processes, including wound healing, tissue repair, and embryonic development . KGF-2 is expressed in the limb mesenchyme and is induced shortly after skin injury, promoting re-epithelialization and tissue regeneration .

Applications

The recombinant form of KGF-2, tagged with His, is widely used in research and therapeutic applications. It is utilized in studies related to wound healing, tissue engineering, and regenerative medicine. The His tag allows for easy purification and detection, making it a valuable tool for researchers .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

KGF 2 Human
Keratinocyte Growth Factor-2 Human Recombinant
Cat. No.
BT19538
Source
Escherichia Coli.
KGF 2 Mouse
Keratinocyte Growth Factor-2 Mouse Recombinant
Cat. No.
BT19617
Source
Escherichia Coli.
KGF 2 Rat
Keratinocyte Growth Factor-2 Rat Recombinant
Cat. No.
BT19683
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.