KGF 2 Rat

Keratinocyte Growth Factor-2 Rat Recombinant
Cat. No.
BT19683
Source
Escherichia Coli.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KGF 2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.0kDa.
The KGF 2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Keratinocyte growth factor 2 (KGF-2), also known as fibroblast growth factor 10 (FGF-10), belongs to the fibroblast growth factor (FGF) family. FGFs are known for their roles in promoting cell growth and survival. They participate in various biological processes, including embryonic development, cell growth regulation, tissue formation, tissue repair, and have implications in tumor development and spread. KGF-2 specifically stimulates the growth of keratinizing epidermal cells, with minimal effect on fibroblasts, a characteristic shared with FGF-7. Studies on the mouse counterpart of the KGF-2 gene suggest its crucial role in the formation of the epidermis during embryonic development. This includes brain development, lung formation, and the initiation of limb bud development. Furthermore, KGF-2 is believed to play a critical role in wound healing.
Description
Recombinant Rat KGF-2, produced in E. coli, is a single-chain polypeptide that lacks glycosylation. It consists of 179 amino acids and has a molecular weight of 20.0 kDa. The purification of KGF-2 is achieved through proprietary chromatographic methods.
Physical Appearance
White, lyophilized powder, sterile-filtered.
Formulation
The product is lyophilized from a 0.2 µm filtered solution, concentrated in 1X PBS (pH 7.4) and containing 5% trehalose.
Solubility
To reconstitute the lyophilized Rat KGF-2, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized KGF-2, though stable at room temperature for 3 weeks, should be stored in dry conditions below -18°C. Once reconstituted, KGF-2 should be kept at 4°C for a period of 2-7 days. For long-term storage, it is advised to add a carrier protein such as HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 97.0%, as determined by: (a) RP-HPLC analysis, (b) SDS-PAGE analysis.
Biological Activity
The biological activity is comparable to the standard, demonstrating full activity. The ED50, measured by the dose-dependent increase in thymidine uptake by BaF3 cells expressing FGF receptors, is less than 0.5 ng/ml.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Source
Escherichia Coli.
Amino Acid Sequence
QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Product Science Overview

Biological Properties and Functions

KGF-2 is known for its proliferative effects on epithelial cells. It is synthesized by stromal cells from epithelial organs and interacts with the FGFR2iiib receptor . The gene encoding KGF-2 maps to the 5p12-p13 region and produces a protein consisting of 208 amino acids. This protein shows a high degree of homology to rat FGF-10 (95.6%), mouse FGF-10 (92%), and human KGF-1 (57%) .

Mode of Action

KGF-2 specifically targets epidermal keratinocytes in vitro and is induced within 24 hours after skin injury in normal animals . It is also expressed in the limb mesenchyme and is involved in the wound healing process . In mouse cutaneous wounds, KGF-2 mRNA is induced one day after injury and rapidly decreases thereafter .

Therapeutic Applications

KGF-2 has been studied for its therapeutic effects in various conditions. For instance, recombinant human KGF-2 (rhKGF-2) has shown potential in promoting lung growth and development, reducing pulmonary edema, and decreasing inflammation in a hyperoxia-induced bronchopulmonary dysplasia (BPD) rat model . This suggests that KGF-2 could be a valuable therapeutic agent for treating lung injuries and other related conditions.

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