KGF 2 Mouse

Keratinocyte Growth Factor-2 Mouse Recombinant
Cat. No.
BT19617
Source
Escherichia Coli.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KGF 2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 19.5kDa.
The KGF 2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Keratinocyte growth factor 2 (KGF-2), also known as fibroblast growth factor 10 (FGF-10), is a member of the fibroblast growth factor (FGF) family. FGFs are signaling molecules with diverse roles in cell growth, survival, and differentiation. KGF-2 specifically stimulates the proliferation and differentiation of epithelial cells, particularly keratinocytes in the skin. It plays a crucial role in wound healing, embryonic development, and tissue homeostasis.
Description
Recombinant mouse KGF-2 is a single, non-glycosylated polypeptide chain produced in E. coli. It contains 173 amino acids and has a molecular weight of 19.5 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile white lyophilized (freeze-dried) powder.
Formulation
The protein is lyophilized from a 0.2 µm filtered solution in 1x phosphate-buffered saline (PBS), pH 7.4 containing 5% trehalose.
Solubility
To reconstitute lyophilized mouse KGF-2, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized KGF-2 is stable at room temperature for up to 3 weeks. For long-term storage, it is recommended to store the desiccated protein below -18°C. Upon reconstitution, KGF-2 should be stored at 4°C for 2-7 days or below -18°C for longer periods. The addition of a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 97.0%, as determined by reverse-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of the recombinant mouse KGF-2 is comparable to the standard. The ED50, measured by the dose-dependent stimulation of thymidine uptake in BaF3 cells expressing FGF receptors, is less than 0.5 ng/ml.
Synonyms
FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Source
Escherichia Coli.
Amino Acid Sequence
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT

Product Science Overview

Introduction

Keratinocyte Growth Factor-2 (KGF-2), also known as Fibroblast Growth Factor 10 (FGF-10), is a member of the fibroblast growth factor (FGF) family. This family consists of at least 23 related peptides that play crucial roles in various biological processes, including cell growth, development, and tissue repair . KGF-2 is particularly known for its role in stimulating the proliferation and differentiation of epithelial cells, making it a significant factor in wound healing and tissue regeneration .

Structure and Function

KGF-2 is produced by fibroblasts in the dermis, granulation tissue, and intraepithelial cells. It specifically acts on cells of epithelial origin, such as skin keratinocytes and intestinal epithelial cells, by binding to the FGF receptor 2-IIIb (FGFR2-IIIb) . The protein sequence of KGF-2 shows high homology with other members of the FGF family, particularly FGF-7 (KGF-1), indicating similar biological functions .

Biological Role

KGF-2 plays a pivotal role in various biological processes:

  • Wound Healing: KGF-2 is involved in the repair of cutaneous wounds by stimulating keratinocyte migration and proliferation. It is rapidly induced after skin injury and contributes to the re-epithelialization process .
  • Hair Growth: KGF-2 is found in dermal papilla fibroblasts and its receptor, FGFR2, is present in the outer root sheath of keratinocytes. This interaction promotes hair growth by stimulating the proliferation of hair follicle cells .
  • Gastrointestinal Epithelium Repair: KGF-2 enhances the healing of mucosal wounds in the gastrointestinal tract by promoting the growth and migration of intestinal epithelial cells .
Recombinant Production

The recombinant production of KGF-2 involves the expression and purification of the protein using various biotechnological techniques. One common method is the fusion of KGF-2 with a small ubiquitin-related modifier (SUMO) to enhance protein expression and prevent degradation . The fusion protein is then purified using affinity chromatography, and the SUMO tag is removed to obtain the recombinant KGF-2 .

Applications

KGF-2 has several potential applications in medical and cosmetic fields:

  • Therapeutic Use: Due to its role in wound healing and tissue regeneration, KGF-2 is being explored as a therapeutic agent for treating chronic wounds, burns, and other skin injuries .
  • Cosmetic Use: KGF-2 is also being investigated for its potential in promoting hair growth and improving skin health, making it a valuable ingredient in cosmetic products .

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