KGF Mouse
Keratinocye Growth Factor Mouse Recombinant
Cat. No.
BT19962
Source
Escherichia Coli.
Synonyms
HBGF-7, FGF7, FGF-7, KGF, Keratinocyte growth factor, Fibroblast growth factor 7, Heparin-binding growth factor 7.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Keratinocyte Growth Factor-1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa.
The FGF-7 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), belongs to the fibroblast growth factor (FGF) family. FGFs are known for their roles in cell growth, survival, and various biological processes, including embryonic development, tissue repair, and tumor progression. KGF specifically stimulates the growth and proliferation of epithelial cells, particularly keratinocytes, while having minimal impact on fibroblasts and endothelial cells. Studies have shown its involvement in epithelial morphogenesis, wound healing, hair follicle development, and early lung development.
Description
Recombinant Mouse Keratinocyte Growth Factor-1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 164 amino acids, resulting in a molecular weight of 18.9 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile.
Formulation
The protein was lyophilized from a solution containing 20mM Phosphate buffer (pH 8) and 0.1M NaCl at a concentration of 1mg/ml.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized KGF in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized KGF remains stable for up to 3 weeks at room temperature. However, for long-term storage, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, the FGF7 solution should be stored at 4°C and used within 2-7 days. For extended storage periods, adding a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% using the following methods: (a) High-Performance Liquid Chromatography (HPLC) analysis and (b) SDS-PAGE analysis.
Biological Activity
The ED50, which represents the effective concentration for half-maximal response, is determined to be less than 10ng/ml. This value is determined by measuring the dose-dependent stimulation of KGF-responsive BaF3 indicator cells using 3H-thymidine uptake as a measure of cell proliferation. This corresponds to a specific activity of 100,000 Units/mg.
Synonyms
HBGF-7, FGF7, FGF-7, KGF, Keratinocyte growth factor, Fibroblast growth factor 7, Heparin-binding growth factor 7.
Source
Escherichia Coli.
Amino Acid Sequence
MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT.
Product Science Overview
Discovery and Function
KGF was first identified due to its potent mitogenic effects on epithelial cells, particularly keratinocytes. Unlike other fibroblast growth factors, KGF’s activity is predominantly restricted to epithelial cells, making it a paracrine growth factor. This means that KGF is produced by mesenchymal cells and acts on nearby epithelial cells .
Biological Significance
KGF plays a significant role in:
- Wound Healing: It promotes the proliferation and differentiation of keratinocytes, which are essential for reepithelialization of wounds .
- Development: KGF is involved in the morphogenesis of various epithelial tissues, including the skin, lungs, and gastrointestinal tract .
- Protection: It helps protect epithelial cells from damage caused by chemotherapy, radiation, and other insults .
Recombinant KGF
Stability and Mutagenesis
One of the challenges with recombinant KGF is its stability. Native KGF tends to be unstable, leading to the formation of aggregates during storage. To address this, researchers have developed various analogs of KGF through mutagenesis. For example, replacing certain cysteine residues with serine has been shown to increase the stability of the protein without compromising its biological activity .
Applications
Recombinant KGF has several applications:
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