IFN gamma Antibody

IFN-γ is a protein with a molecular weight of approximately 15-17 kDa . It exerts several immunoregulatory, anti-proliferative, anti-viral, and pro-inflammatory activities . The production of IFN-γ is associated with the differentiation of T helper 1 (Th1) cells, which are essential for the immune response against intracellular pathogens .

Rat anti-mouse IFN-γ antibodies, such as the XMG1.2 clone, are monoclonal antibodies specifically designed to bind to mouse IFN-γ . These antibodies are commonly used in research to study the expression and function of IFN-γ in various experimental settings. They are particularly useful in techniques like flow cytometry and immunofluorescent staining to identify and enumerate IFN-γ producing cells within mixed cell populations .

1. Flow Cytometry: Rat anti-mouse IFN-γ antibodies are used for intracellular staining in flow cytometric analysis. This technique helps in identifying and quantifying IFN-γ producing cells in a sample .
2. Immunofluorescent Staining: These antibodies are also employed in immunofluorescent staining protocols to visualize IFN-γ expression in cells and tissues .
3. Neutralization Studies: The purified form of these antibodies has been reported to act as neutralizing antibodies, which can inhibit the biological activity of IFN-γ in various assays .

Rat anti-mouse IFN-γ antibodies should be stored undiluted at 4°C and protected from prolonged exposure to light. They should not be frozen to maintain their stability and functionality .