IFN g Mouse
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
IFN-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Product Specs
When lymphocytes are activated by specific antigens or mitogens, they produce IFN-gamma. This cytokine possesses significant immunoregulatory properties and acts as a potent activator of macrophages. Furthermore, IFN-gamma exhibits antiviral activity, antiproliferative effects on transformed cells, and the ability to enhance the antiviral and antitumor effects of type I IFNs.
Recombinant Mouse IFN-gamma, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 134 amino acids. With a molecular weight of 15.6 kDa, this IFN-gamma undergoes purification using proprietary chromatographic techniques.
To reconstitute the lyophilized IFN-gamma, it is recommended to use sterile distilled water or 20 mM acetic acid (AcOH) at concentrations between 0.1 mg/mL and 0.5 mg/mL. Once reconstituted, it can be further diluted in other aqueous solutions.
While lyophilized IFN-gamma remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, IFN-gamma should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
The specific activity, determined using a viral resistance assay, is less than 0.1 ng/mL, which corresponds to a specific activity of 10,000,000 IU/mg.
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Product Science Overview
IFN-γ has several important biological functions:
- Antiviral Activity: It demonstrates significant antiviral activity, as evidenced by its ability to inhibit the growth of viruses in infected cells .
- Immunoregulatory Functions: IFN-γ plays a key role in regulating the immune response. It activates the cytotoxic activity of innate immune cells such as macrophages and NK cells .
- Inflammatory Response: It is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells .
Through cell signaling, IFN-γ regulates the immune response of its target cells. A key signaling pathway activated by type II IFN is the JAK-STAT signaling pathway . By activating signaling pathways in cells such as macrophages, B cells, and CD8+ cytotoxic T cells, IFN-γ promotes inflammation, antiviral or antibacterial activity, and cell proliferation and differentiation .
