IFN g Rat
IFN-Gamma Rat Recombinant
Cat. No.
BT26852
Source
Escherichia Coli.
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
IFN-gamma Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 135 amino acids and having a molecular mass of 15609 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interferon gamma (IFN-gamma) is a cytokine produced by activated lymphocytes, such as T cells and natural killer (NK) cells, in response to antigenic or mitogenic stimulation. It exhibits antiviral properties and plays a crucial role in immune regulation. IFN-gamma acts as a potent activator of macrophages, enhancing their phagocytic and antimicrobial activities. Furthermore, it exerts antiproliferative effects on transformed cells and can augment the antiviral and antitumor effects of type I interferons.
Description
Recombinant Rat IFN-gamma, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 135 amino acids. With a molecular weight of 15.6 kDa (15,609 Daltons), this IFN-gamma variant is purified using proprietary chromatographic techniques to ensure high purity and biological activity.
Physical Appearance
Sterile Filtered White Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered concentrated (1 mg/mL) solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
Reconstitute the lyophilized IFN-gamma in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/mL. Further dilutions can be made in other aqueous solutions as needed.
Stability
Lyophilized IFN-gamma remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product desiccated at temperatures below -18°C. Upon reconstitution, IFN-gamma should be stored at 4°C for 2-7 days. For extended storage, freeze at -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity > 95.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The specific activity, determined using a cytopathic effect inhibition assay with murine L929 cells challenged with encephalomyocarditis virus (EMCV), was less than 0.1 ng/mL. This corresponds to a specific activity of 10,000,000 units/mg.
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Gly-Tyr-Leu.
Product Science Overview
Structure and Production
Recombinant rat IFN-γ is typically produced using E. coli expression systems. The protein consists of 134 amino acids with a molecular weight of approximately 15.5 kDa . It is often produced in a carrier-free form to avoid interference from other proteins, such as bovine serum albumin (BSA), which is sometimes used to enhance protein stability .
Biological Functions
IFN-γ is a key player in both innate and adaptive immunity. It exerts a wide range of immunoregulatory activities, including:
- Activation of Macrophages: Enhances the microbicidal activity of macrophages, aiding in the clearance of pathogens .
- Antiviral and Antibacterial Activity: Promotes the expression of genes involved in antiviral and antibacterial responses .
- Regulation of Immune Cells: Influences the differentiation and proliferation of various immune cells, including T cells and B cells .
- Proinflammatory Cytokine: Acts as a prototype proinflammatory cytokine, promoting inflammation to combat infections .
Applications in Research
Stability and Storage
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