IFNG Feline
Interferon-gamma Feline Recombinant
Cat. No.
BT27882
Source
Escherichia Coli.
Synonyms
Interferon gamma, IFN-gamma, IFNG.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IFNG Feline Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (24-167 a.a) and having a molecular mass of 19.3kDa.
IFNG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
IFNG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interferon gamma (IFN-gamma) is a cytokine produced by activated lymphocytes, such as T cells and natural killer cells, in response to antigenic or mitogenic stimulation. IFN-gamma exhibits antiviral properties and plays a crucial role in immune regulation. It acts as a potent activator of macrophages, enhancing their ability to eliminate pathogens and tumor cells. Moreover, IFN-gamma demonstrates antiproliferative effects on transformed cells and can augment the antiviral and antitumor activities of type I interferons.
Description
Recombinant Feline Interferon Gamma (IFNG) is a non-glycosylated protein produced in E. coli. It consists of a single polypeptide chain comprising 167 amino acids, with a molecular weight of 19.3 kDa. The protein sequence encompasses amino acids 24 to 167 of the mature IFNG protein. A 23-amino acid Histidine tag is fused to the N-terminus to facilitate purification. The recombinant IFNG undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized through filtration.
Formulation
The IFNG protein is supplied in a solution containing 1 mg/ml of IFNG, phosphate-buffered saline (PBS) at pH 7.4, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept refrigerated at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. To preserve protein stability during long-term storage, the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advised. Avoid repeated freezing and thawing of the product.
Purity
The purity of the IFNG protein is determined to be greater than 95% using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
Interferon gamma, IFN-gamma, IFNG.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQAMFFKE IEELKGYFNA SNPDVADGGS LFVDILKNWK EESDKTIIQS QIVSFYLKMF ENLKDDDQRI QRSMDTIKED MLDKLLNTSS SKRDDFLKLI QIPVNDLQVQ RKAINELFKV MNDLSPRSNL RKRKRSQNLF RGRRASK.
Product Science Overview
Structure and Function
Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits . This cytokine exerts several important functions:
- Antiviral Activity: IFN-gamma has potent antiviral properties, helping to inhibit viral replication within host cells.
- Immunoregulatory Functions: It is a potent activator of macrophages, enhancing their ability to phagocytose pathogens and present antigens. It also promotes the differentiation of T helper cells and enhances the cytotoxic activity of natural killer cells .
- Antiproliferative Effects: IFN-gamma can inhibit the proliferation of transformed cells and potentiate the antiviral and antitumor effects of type I interferons .
Recombinant Feline IFN-gamma
Recombinant feline IFN-gamma is produced using genetic engineering techniques, where the gene encoding feline IFN-gamma is inserted into a host organism, such as Escherichia coli, to produce the protein in large quantities . This recombinant protein is used in various research and therapeutic applications.
Production and Purification:
- The recombinant protein is typically expressed in E. coli and purified using chromatographic techniques to achieve high purity levels (>95%) as determined by SDS-PAGE .
- The endotoxin level is kept below 0.01 EU per 1 μg of the protein to ensure its safety for use in biological assays .
Applications:
- Research: Recombinant feline IFN-gamma is used in various in vitro and in vivo studies to understand its role in immune responses and its potential therapeutic applications.
- Therapeutics: It has potential applications in treating viral infections and certain types of cancer in felines. Its antiviral and immunomodulatory properties make it a valuable tool in veterinary medicine .
Stability and Storage
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