IFN g Human
IFN-Gamma Human Recombinant
Cat. No.
BT26617
Source
Escherichia Coli.
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
IFN-gamma Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Product Specs
Introduction
IFN-gamma, also known as interferon-gamma, is a cytokine primarily produced by activated lymphocytes, including T cells and natural killer cells. This cytokine plays a crucial role in both innate and adaptive immunity. Its primary function is to activate macrophages, enhancing their ability to kill intracellular pathogens. Additionally, IFN-gamma exhibits antiviral activity, inhibits the proliferation of transformed cells, and synergizes with type I interferons to enhance antiviral and antitumor effects.
Description
Recombinant Human IFN-gamma, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 144 amino acids. With a molecular weight of 17 kDa, this protein is purified using proprietary chromatographic techniques, ensuring high purity and biological activity.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 4.6.
Solubility
To reconstitute the lyophilized IFN-gamma, it is recommended to dissolve it in sterile distilled water or 20mM acetic acid (AcOH) to a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IFN-gamma remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized protein desiccated at a temperature below -18°C. After reconstitution, IFN-gamma should be stored at 4°C for 2-7 days. For prolonged storage, freezing at -18°C is recommended. To ensure optimal stability during long-term storage, the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advised. Avoid repeated freeze-thaw cycles to maintain protein integrity and activity.
Purity
The purity of IFN-gamma is determined using two methods: reverse-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purity is greater than 98.0% as determined by both methods.
Biological Activity
The biological activity of IFN-gamma is determined using a viral resistance assay. The specific activity is less than 0.05 ng/ml, which corresponds to a specific activity of 2.0 x 10,000,000 international units (IU) per milligram (mg).
Protein Content
The protein content of IFN-gamma is quantified using two independent methods. The first method involves UV spectroscopy at 280 nm, employing an absorbance value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is derived from the analysis of protein sequences using the PC GENE computer program (IntelliGenetics). The second method utilizes RP-HPLC, where a calibrated solution of IFN-gamma serves as a reference standard for accurate quantification.
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Source
Escherichia Coli.
Amino Acid Sequence
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI
VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS
VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.
Product Science Overview
Discovery and Production
Biological Functions
IFN-γ is a potent multifunctional cytokine with several key roles:
- Immune Regulation: It regulates the immune response by activating macrophages, enhancing antigen presentation, and promoting the differentiation of T helper cells .
- Antiviral and Antibacterial Activity: IFN-γ has antiviral properties and can inhibit the growth of certain bacteria .
- Inflammation: It promotes inflammation by activating various immune cells and increasing the production of pro-inflammatory cytokines .
Recombinant IFN-Gamma
Clinical Applications
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