IFNG Mouse, His

Interferon-gamma Mouse Recombinant, His Tag
Cat. No.
BT28250
Source
Escherichia Coli.
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (23-155a.a.) and having a molecular mass of 18.2kDa.
IFNG is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
IFN-gamma is produced by lymphocytes when activated by specific antigens or mitogens. This cytokine plays a critical role in the immune response, exhibiting antiviral activity and significant immunoregulatory functions. Notably, IFN-gamma acts as a potent activator of macrophages, demonstrates antiproliferative effects on transformed cells, and can enhance the antiviral and antitumor activities of type I interferons.
Description
Recombinant Mouse Interferon-gamma (IFNG), expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 158 amino acids (specifically residues 23-155) and has a molecular weight of 18.2kDa. The IFNG protein includes a 25 amino acid His-tag fused to its N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The IFNG protein solution is provided at a concentration of 0.5mg/ml. The solution contains the following components: 20mM MES buffer (pH 5.0), 1mM DTT, 0.2M NaCl, and 40% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if the entire vial will be used within 2-4 weeks. For longer-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein like 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity of the IFNG protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMHGTVI ESLESLNNYF NSSGIDVEEK SLFLDIWRNW QKDGDMKILQ SQIISFYLRL FEVLKDNQAI SNNISVIESH LITTFFSNSK AKKDAFMSIA KFEVNNPQVQ RQAFNELIRV VHQLLPESSL RKRKRSRC.

Product Science Overview

Introduction

Interferon-gamma (IFN-γ) is a critical cytokine belonging to the type II interferon family. It plays a pivotal role in the immune response, particularly in antiviral, immunoregulatory, and anti-tumor activities. The recombinant form of mouse IFN-γ, tagged with a polyhistidine (His) tag, is widely used in research to study its biological functions and interactions.

Molecular Characteristics

The recombinant mouse IFN-γ protein is typically produced in HEK293 cells, a human embryonic kidney cell line, which ensures proper folding and post-translational modifications. The protein consists of 144 amino acids and has a calculated molecular mass of approximately 17 kDa. Due to glycosylation, its apparent molecular mass is around 21 kDa when analyzed by SDS-PAGE under reducing conditions .

Expression and Purification

The DNA sequence encoding mouse IFN-γ (accession number P01580) is expressed with a polyhistidine tag at the C-terminus. This His tag facilitates the purification of the protein using immobilized metal affinity chromatography (IMAC), which exploits the affinity between histidine residues and metal ions like nickel or cobalt .

Biological Activity

Recombinant mouse IFN-γ exhibits significant biological activity. It is measured in antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV), where the effective dose (ED50) typically ranges from 0.2 to 1 ng/mL. Additionally, its binding ability is assessed through functional ELISA, demonstrating interaction with mouse IFN-γ receptor 1 (IFNGR1) fused with an Fc tag .

Functional Significance

IFN-γ is predominantly produced by natural killer (NK) cells, natural killer T (NKT) cells, and effector T cells (CD4+ and CD8+). It is a key player in the innate and adaptive immune responses. IFN-γ activates macrophages, enhances antigen presentation, and promotes the differentiation of T helper 1 (Th1) cells. It also has direct antiviral effects and can inhibit the proliferation of certain tumor cells .

Applications in Research

The recombinant mouse IFN-γ protein is extensively used in immunological research to:

  • Study cytokine signaling pathways.
  • Investigate the mechanisms of immune cell activation and differentiation.
  • Evaluate the antiviral and anti-tumor properties of IFN-γ.
  • Develop therapeutic strategies for diseases involving immune dysregulation.

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