IFN g Equine
Interferon-Gamma Equine Recombinant
Cat. No.
BT26494
Source
Escherichia Coli.
Synonyms
Interferon gamma, IFN-gamma, IFNG.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Recombinant Equine Interferon-gamma produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 16.7kDa.
The IFN-gamma Equine is purified by proprietary chromatographic techniques.
The IFN-gamma Equine is purified by proprietary chromatographic techniques.
Product Specs
Introduction
IFN-gamma is a cytokine primarily produced by activated lymphocytes, including T cells and natural killer cells, in response to antigenic or mitogenic stimulation. This cytokine plays a crucial role in both innate and adaptive immunity. Beyond its antiviral properties, IFN-gamma exhibits potent immunomodulatory functions, such as activating macrophages, inhibiting the proliferation of transformed cells, and enhancing the antitumor and antiviral effects of type I interferons.
Description
Recombinant Equine Interferon-gamma, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 143 amino acids. With a molecular weight of 16.7 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, white powder obtained through lyophilization (freeze-drying).
Formulation
The lyophilization process involves a 0.2 µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.4 with 5% trehalose.
Solubility
For reconstitution, dissolve the lyophilized Interferon-gamma in sterile 18 MΩ·cm H₂O to a concentration of at least 100 µg/ml. Further dilutions can be made in other aqueous solutions.
Stability
Lyophilized Interferon-gamma remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, store IFN-gamma at 4°C for 2-7 days. For long-term storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined using the following methods: (a) Analysis by reversed-phase high-performance liquid chromatography (RP-HPLC) and (b) Analysis by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purity is greater than 97.0%.
Biological Activity
The biological activity is determined using an anti-viral assay with human HeLa cells infected with the encephalomyocarditis (EMC) virus. The half-maximal effective concentration (ED₅₀) is less than 10.0 ng/ml, which corresponds to a specific activity greater than 1.0 x 10⁵ IU/mg.
Synonyms
Interferon gamma, IFN-gamma, IFNG.
Source
Escherichia Coli.
Amino Acid Sequence
QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQKLIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ.
Product Science Overview
Structure and Function
Mature equine IFN-γ exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits . This cytokine exerts a wide range of immunoregulatory activities, including:
- Activation of macrophages: Enhancing their ability to phagocytose pathogens.
- Induction of antigen presentation: Increasing the expression of MHC class I and II molecules.
- Promotion of Th1 cell differentiation: Encouraging the development of T-helper cells that produce more IFN-γ, thus amplifying the immune response.
Recombinant Production
Recombinant equine IFN-γ is produced using E. coli expression systems . The recombinant protein is typically purified to a high degree of purity (>97%) and is available in both carrier-free and carrier-containing formulations . The carrier protein, often Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .
Applications
Recombinant equine IFN-γ is used in various research and clinical applications, including:
- Antiviral assays: Measuring its activity in inhibiting viral replication in cell cultures .
- Immunological studies: Investigating its role in immune responses and its potential therapeutic applications.
- Veterinary medicine: Exploring its use in treating equine diseases and enhancing immune responses in horses.
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