IFNG Canine

Interferon-gamma Canine Recombinant
Cat. No.
BT27713
Source
Escherichia Coli.
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Recombinant IFNG Dog produced in E.coli cells is a non-glycosylated, homodimeric protein containing 143 amino acid chain and having a molecular mass of 16.9kDa. IFNG is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IFN-gamma is a cytokine primarily produced by activated lymphocytes, including T cells and natural killer (NK) cells, in response to specific antigens or mitogens. This cytokine exhibits antiviral properties and plays a crucial role in immune regulation. IFN-gamma acts as a potent activator of macrophages, exerts antiproliferative effects on transformed cells, and enhances the antiviral and antitumor activities of type I interferons.
Description
Recombinant Canine IFNG, expressed in E. coli, is a non-glycosylated, homodimeric protein. It consists of two identical polypeptide chains, each comprising 143 amino acids, resulting in a molecular weight of 16.9 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The IFNG was lyophilized from a 0.2 µm filtered solution containing 25mM Sodium Succinate (pH 5.0), 60mM NaCl, and 0.1% Tween-80.
Solubility
To reconstitute the lyophilized IFNG, it is recommended to dissolve it in sterile distilled water at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IFNG remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at temperatures below -18°C for optimal long-term stability. Once reconstituted, IFNG should be stored at 4°C for 2-7 days. For extended storage, freezing at temperatures below -18°C is recommended. To enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of IFNG is determined using two methods: RP-HPLC and SDS-PAGE analysis. The results from both methods indicate a purity greater than 97.0%.
Biological Activity
The biological activity of IFNG is determined through an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV). The ED50, which represents the effective concentration required to inhibit viral replication by 50%, is less than 2.0 ng/ml. This corresponds to a specific activity exceeding 5.0 x 10⁵ IU/mg.
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Source
Escherichia Coli.
Amino Acid Sequence
QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK

Product Science Overview

Structure and Production

Recombinant Canine Interferon-gamma (rCaIFN-gamma) is a non-glycosylated polypeptide chain containing 143 amino acids. It is produced using Escherichia coli (E. coli) as the expression system . The mature form of canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits .

Biological Activity

The biological activity of recombinant canine IFN-gamma is measured by its antiviral activity on A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV). The effective dose (ED50) for this effect is typically in the range of 0.3-2.0 ng/mL . This cytokine is fully biologically active when compared to the standard, with a specific activity of greater than 5.0 x 10^5 IU/mg .

Applications and Uses

Recombinant canine IFN-gamma is used in various research and clinical applications, including:

  • Immunotherapy: Enhancing the immune response against infections and tumors.
  • Research: Studying the mechanisms of immune regulation and cytokine signaling.
  • Veterinary Medicine: Potential therapeutic applications in treating canine diseases.
Storage and Stability

The recombinant protein is typically lyophilized from a filtered solution and can be reconstituted in sterile phosphate-buffered saline (PBS) containing at least 0.1% human or bovine serum albumin. It is stable for 12 months when stored at -20 to -70°C as supplied. After reconstitution, it can be stored for 1 month at 2-8°C under sterile conditions and for 3 months at -20 to -70°C under sterile conditions .

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