IFN g Antibody

Interferon-gamma, Mouse Anti-Human
Cat. No.
BT6064
Source
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Product Specs

Introduction
Interferon gamma (IFN-gamma) is a cytokine primarily produced by activated lymphocytes, such as T cells and natural killer (NK) cells, in response to antigenic or mitogenic stimulation. This cytokine plays a crucial role in both innate and adaptive immune responses, exhibiting a wide range of biological activities. Primarily known for its antiviral properties, IFN-gamma effectively inhibits viral replication within host cells. Moreover, it serves as a potent activator of macrophages, enhancing their phagocytic and antigen-presenting capabilities, thereby bolstering the immune system's ability to eliminate pathogens. Furthermore, IFN-gamma demonstrates antiproliferative effects on transformed cells, potentially contributing to tumor suppression. Notably, it can synergize with type I interferons, amplifying their antiviral and antitumor effects. Overall, IFN-gamma is a pleiotropic cytokine with critical implications in immune regulation and host defense mechanisms.
Formulation
The antibody is supplied as a solution with a concentration of 1 milligram per milliliter in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
To ensure stability during transportation, the antibody is lyophilized and shipped at ambient temperature.
Storage Procedures
For long-term storage, it is recommended to keep the lyophilized antibody at a temperature of 4 degrees Celsius in a dry environment. Following reconstitution, if the antibody is not intended for immediate use (within one month), it is advisable to aliquot and store it at -20 degrees Celsius to maintain its activity.
Solubility
To reconstitute the lyophilized antibody, add sterile water (H2O) to the vial. Gently mix the solution by swirling or inverting the vial to ensure complete dissolution. Avoid vigorous shaking to prevent protein denaturation. Rinse the sides of the vial with the reconstituted solution and allow it to sit undisturbed for 30-60 seconds before use.
Titer
Immobilization of this antibody on plastic surfaces results in a loss of its ability to bind to its target antigen. The biological activity of this antibody is assessed through its capacity to neutralize viruses and induce the expression of major histocompatibility complex class II (MHC class II) molecules.
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Purification Method
Ion exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYRhIFNg.
Immunogen
r.Human IFN-g.
Ig Subclass
Mouse IgG2a.

Product Science Overview

Structure and Function

IFN-γ is a glycoprotein composed of 143 amino acid residues, with a molecular weight ranging between 14-18 kDa . It is known for its ability to upregulate MHC class I and II antigen expression by antigen-presenting cells, enhancing the immune system’s ability to recognize and respond to pathogens .

Production and Secretion

The production of IFN-γ is primarily induced in response to antigenic stimulation. It is produced by several activated cell types, including NK cells, NKT cells, CD4+ T cells, CD8+ T cells, and TCRγδ+ T cells . The secretion of IFN-γ is a critical component of the immune response, particularly in the context of viral infections and tumor surveillance .

Mouse Anti-Human IFN-γ Antibody

The Mouse Anti-Human IFN-γ antibody is a monoclonal antibody that specifically binds to human IFN-γ. This antibody is typically produced by immunizing mice with recombinant human IFN-γ expressed in E. coli . The antibody is then purified using affinity chromatography to ensure high specificity and purity .

Applications

The Mouse Anti-Human IFN-γ antibody is widely used in various scientific applications, including:

  • Flow Cytometry: For intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations .
  • ELISA: To quantify IFN-γ levels in biological samples .
  • Western Blot: For detecting IFN-γ protein in cell lysates .
  • Immunohistochemistry and Immunocytochemistry: For localizing IFN-γ in tissue sections and cell preparations .
Importance in Research

The Mouse Anti-Human IFN-γ antibody is a valuable tool in immunological research. It allows scientists to study the production, regulation, and function of IFN-γ in various physiological and pathological contexts. This antibody is particularly useful in research related to infectious diseases, cancer immunotherapy, and autoimmune disorders .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IFN gamma Antibody
Interferon-gamma, Rat Anti-Mouse
Cat. No.
BT2455
Source
IFN g Equine
Interferon-Gamma Equine Recombinant
Cat. No.
BT26494
Source
Escherichia Coli.
IFN g Human
IFN-Gamma Human Recombinant
Cat. No.
BT26617
Source
Escherichia Coli.
IFN g Human, His
Interferon-Gamma Human Recombinant, His Tag
Cat. No.
BT26692
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.