IL 4 Antibody
Interleukin-4, Rat Anti-Mouse
Cat. No.
BT2752
Source
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Interleukin 4 (IL-4) is a pleiotropic cytokine with a crucial role in immune responses. Primarily produced by activated T cells, IL-4 exhibits diverse biological activities. It functions as a ligand for the interleukin 4 receptor, which also binds IL-13, resulting in overlapping functions between these two cytokines. Upon binding to its receptor, IL-4 initiates intracellular signaling cascades, with STAT6 (signal transducer and activator of transcription) playing a central role in mediating its immune regulatory effects. The gene encoding IL-4 is located on chromosome 5q within a cytokine gene cluster comprising IL3, IL5, IL13, and CSF2, with a particularly close proximity to IL13. Notably, the expression of IL-4, IL-13, and IL-5 is coordinately regulated by multiple long-range regulatory elements spanning over 120 kilobases on the chromosome. Alternative splicing of the IL-4 gene generates two transcript variants encoding distinct isoforms, further contributing to the functional diversity of this cytokine.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) following reconstitution.
Shipping Conditions
To maintain stability during transport, the antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, the lyophilized antibody should be kept at 4°C in a dry environment. After reconstitution, it is recommended to aliquot the antibody and store it at -20°C if not intended for use within one month.
Solubility
To reconstitute the lyophilized antibody, add sterile water (H2O). Gently mix the solution by swirling or inverting the vial. Ensure that the contents are thoroughly dissolved and avoid excessive agitation. Allow the solution to sit for 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for a variety of applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation, immunohistochemistry, and intracellular staining.
Titer
In a direct ELISA setup, a 1:5,000 dilution of this antibody yields an optical density (O.D.) of 0.4 when using an alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (IgG) secondary antibody from Jackson Laboratories.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Purification Method
Ion exchange.
Type
Rat Anti Mouse Monoclonal.
Clone
NYRmIL-4.
Immunogen
r.Murine IL-4.
Ig Subclass
Rat IgG1.
Product Science Overview
Introduction
Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in the immune system. It is primarily produced by activated CD4+ T cells, basophils, and mast cells . IL-4 is involved in various biological processes, including the differentiation of naïve helper T cells (Th0 cells) to Th2 cells, stimulating activated B-cell and T-cell proliferation, and promoting immunoglobulin class switching to IgG1 and IgE in mouse B-cells .
Rat Anti-Mouse IL-4 Antibody
The Rat Anti-Mouse IL-4 antibody is a monoclonal antibody specifically designed to target and neutralize mouse IL-4. This antibody is commonly used in research to study the role of IL-4 in various immunological processes. The immunogen used to generate this antibody is typically recombinant mouse IL-4 .
Applications
The Rat Anti-Mouse IL-4 antibody has several applications in immunological research:
- ELISA (Enzyme-Linked Immunosorbent Assay): It is used as a capture antibody in sandwich ELISA to measure mouse IL-4 protein levels .
- Immunocytochemistry: This antibody can be used to identify and enumerate IL-4 producing cells by immunocytochemistry .
- Neutralization: The antibody is also used to neutralize the activity of IL-4 in various experimental setups .
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