IL 4 Rat
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Interleukin-4 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids and having a molecular mass of 14kDa.
The IL-4 is purified by proprietary chromatographic techniques.
Product Specs
HGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.
Product Science Overview
Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in the immune system. It is also known as B cell-stimulatory factor-1 (BSF-1) or B cell growth factor-1 (BCGF-1) . Recombinant IL-4 from rats is often used in research to study its effects and mechanisms in various biological processes.
IL-4 is a monomeric glycoprotein with a molecular weight ranging from approximately 13 kDa to 18 kDa . It contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure . The recombinant form of rat IL-4 is typically produced in E. coli and is a non-glycosylated polypeptide chain containing 125 amino acids .
IL-4 is primarily produced by activated T cells, mast cells, and bone marrow stromal cells . It induces the differentiation of naive helper T cells (Th0 cells) into Th2 cells, which are essential for humoral immunity . IL-4 also stimulates the proliferation and differentiation of B cells, enhances the expression of MHC class II molecules, and promotes the production of IgE and IgG1 antibodies .
Recombinant rat IL-4 is widely used in immunological research to study its role in various cellular processes. It is used in cell proliferation assays, where it has been shown to stimulate the proliferation of rat splenocytes . Additionally, IL-4 is used to investigate its effects on macrophages, where it has been observed to increase the expression of Arg1 without affecting iNOS or IL-6 .
Recombinant rat IL-4 is produced in E. coli and is available in both carrier-free and carrier-containing formulations . The carrier-free version is recommended for applications where the presence of bovine serum albumin (BSA) could interfere with the results . The purity of recombinant rat IL-4 is typically greater than 95% as determined by SDS-PAGE and HPLC . The endotoxin level is less than 1 EU/μg of the protein, as determined by the LAL method .
Recombinant rat IL-4 is lyophilized from a 0.2 μm filtered solution in PBS and can be reconstituted in sterile distilled water or aqueous buffer containing 0.1% BSA . It is stable for 12 months when stored at -20 to -70°C as supplied . After reconstitution, it can be stored at 2 to 8°C for one month or at -20 to -70°C for long-term storage . It is important to avoid repeated freeze-thaw cycles to maintain its stability and activity .
