IL 4 Mouse
Interleukin-4 Mouse Recombinant
Cat. No.
BT5443
Source
Escherichia Coli.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton.
The IL-4 is purified by proprietary chromatographic techniques.
The IL-4 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-4 (IL-4) is a multifunctional cytokine primarily produced by activated T lymphocytes, basophils, and mast cells. It plays a crucial role in modulating immune responses by acting on various cell types. IL-4 is involved in regulating isotype switching, stimulating IgE production in B lymphocytes, and driving the differentiation of precursor T helper cells. Its effects are mediated through binding to both membrane-bound and soluble IL-4 receptors.
Description
Recombinant Mouse Interleukin-4, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 120 amino acids with a molecular weight of 13.5 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 µM (1 mg/mL) solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized Interleukin-4, it is recommended to dissolve it in sterile 10 mM acetic acid (HAc) at a concentration of at least 100 µg/mL. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-4 is stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, store IL-4 at 4°C for 2-7 days. For extended storage, freeze at -18°C. Avoid repeated freeze-thaw cycles. It is advisable to add a carrier protein (0.1% HSA or BSA) for long-term storage.
Purity
The purity is determined to be greater than 96.0% using the following methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The half-maximal effective concentration (ED₅₀), determined by the dose-dependent induction of HT-2 cell proliferation, is less than 2 ng/mL. This corresponds to a specific activity of 500,000 international units (IU) per mg.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Source
Escherichia Coli.
Amino Acid Sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC
RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES
KSTSLKDFLE SLKSIMQMDY S.
Product Science Overview
Structure and Production
Mouse recombinant IL-4 is a protein that is typically produced using recombinant DNA technology. This involves inserting the gene encoding mouse IL-4 into a suitable expression system, such as Escherichia coli (E. coli) or mammalian cells, to produce the protein in large quantities . The recombinant protein is then purified and lyophilized for use in various applications. The molecular weight of mouse IL-4 is approximately 13.5 kDa, and it consists of 120 amino acid residues .
Biological Functions
IL-4 is a member of the short four-helix bundle family of cytokines and is primarily produced by Th2 cells in response to antigen receptor engagement . It has several important functions in the immune system:
- B-cell Activation and Differentiation: IL-4 stimulates the growth and differentiation of B cells, promoting the production of antibodies .
- T-cell Differentiation: It plays a key role in the differentiation of naive CD4+ T cells into Th2 cells, which are essential for humoral immunity .
- Inhibition of Angiogenesis: IL-4 inhibits VEGF-induced and FGF-basic-induced angiogenesis, which can have implications in cancer and other diseases .
Applications
Recombinant mouse IL-4 is widely used in research and clinical applications. Some of its common uses include:
- Cell Culture: It is used as a component of cell culture media to support the growth and differentiation of immune cells .
- Functional Assays: IL-4 is used in various functional assays to study its effects on immune cells and its potential therapeutic benefits .
- Therapeutic Research: Researchers are investigating the therapeutic potential of IL-4 in treating diseases such as allergies, asthma, and autoimmune disorders .
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