IL 4 Rhesus Macaque

IL-4 is a monomeric protein with a molecular weight ranging from approximately 13 kDa to 18 kDa . The recombinant version of IL-4 from Rhesus Macaque is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 129 amino acids, with a molecular mass of 14.9 kDa . The mature rhesus IL-4 shares 97%, 93%, and 93% amino acid sequence identity with baboon, chimpanzee, and human IL-4, respectively .

IL-4 exerts its effects through two receptor complexes, primarily influencing the differentiation of naive helper T cells (Th0 cells) to Th2 cells . It is involved in the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. IL-4 also enhances the secretion and cell surface expression of IgE and IgG1 .

Recombinant IL-4 from Rhesus Macaque is used in various research applications, including cell proliferation assays, where it has been shown to stimulate the proliferation of TF-1 human erythroleukemic cells . It is also used in studies related to immune responses and cytokine signaling pathways.

The recombinant IL-4 is produced using E. coli expression systems and is purified through proprietary chromatographic techniques . The protein is typically lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. The carrier-free version does not contain BSA, which is recommended for applications where the presence of BSA could interfere .

The recombinant IL-4 is shipped at ambient temperature and should be stored at -20 to -70 °C upon receipt. It is stable for 12 months from the date of receipt when stored as supplied. After reconstitution, it should be stored under sterile conditions at 2 to 8 °C for up to 1 month or at -20 to -70 °C for up to 3 months .