IL 4 Human
Interleukin-4 Human Recombinant
Cat. No.
BT5159
Source
Escherichia Coli.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15kDa.
The IL-4 is purified by proprietary chromatographic techniques.
The IL-4 is purified by proprietary chromatographic techniques.
Product Specs
Description
Recombinant human interleukin-4 (IL-4) is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 130 amino acids with a molecular weight of 15 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile white lyophilized (freeze-dried) powder.
Formulation
IL-4 is lyophilized from a 0.2 μm filtered solution at a concentration of 1 mg/ml in PBS, pH 7.4.
Solubility
To reconstitute the lyophilized IL-4, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 μg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized interleukin-4 remains stable at room temperature for 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, IL-4 can be stored at 4°C for 2-7 days. For future use, store below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% by the following methods:
(a) RP-HPLC analysis
(b) SDS-PAGE analysis
Biological Activity
The ED50, determined by the dose-dependent stimulation of TF-1 cells, is less than 0.2 ng/ml. This corresponds to a specific activity of 5,000,000 IU/mg.
Protein Content
Protein quantification is carried out using two independent methods:
1. UV spectroscopy at 280 nm, employing an absorbance value of 0.594 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated using the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis using a standard solution of IL-4 as a reference standard.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Source
Escherichia Coli.
Amino Acid Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.
Product Science Overview
Biological Functions
- T Cell Differentiation: IL-4 induces the differentiation of naïve helper T cells (Th0) into Th2 cells . Th2 cells are essential for the humoral immune response, which involves the production of antibodies by B cells.
- B Cell Activation: IL-4 promotes the proliferation and differentiation of B cells. It also stimulates immunoglobulin isotype switching, particularly to IgE, which is associated with allergic responses .
- Regulation of Immune Responses: IL-4 plays a central role in humoral and adaptive immune responses. It enhances the expression of MHC class II antigens and low-affinity IgE receptors on B cells .
Recombinant Human IL-4
Recombinant human IL-4 is a laboratory-produced version of the natural cytokine. It is typically produced in E. coli and is used for various research and clinical applications . The recombinant form retains the biological activity of the natural cytokine and is used to study its effects in cell culture and animal models.
Applications:
- Cell Culture: Recombinant IL-4 is used to stimulate the proliferation and differentiation of T and B cells in vitro .
- Functional Assays: It is employed in functional assays to study the role of IL-4 in immune responses and to screen for potential therapeutic agents .
- Differentiation Studies: Researchers use recombinant IL-4 to induce the differentiation of naïve CD4+ T cells into Th2 cells .
Clinical Relevance
IL-4 is associated with various immunological disorders, particularly those involving allergic responses. Excessive production of IL-4 can lead to conditions such as asthma and other IgE-mediated allergies . Understanding the role of IL-4 in these conditions is crucial for developing targeted therapies.
Storage and Handling
Recombinant human IL-4 is typically provided as a lyophilized powder and should be stored at -20°C to -80°C to maintain its stability . Upon reconstitution, it should be aliquoted and stored at 4°C for short-term use or at -20°C for long-term storage . It is important to avoid repeated freeze-thaw cycles to preserve its biological activity.
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