IL 4 Human, Yeast
Interleukin 4 Human Recombinant, Yeast
Cat. No.
BT5355
Source
Pichia pastoris.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.
The IL-4 is purified by proprietary chromatographic techniques.
The IL-4 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin 4 (IL4) is a cytokine that plays a crucial role in regulating immune responses. It is produced by activated T cells and binds to the interleukin 4 receptor, which also interacts with IL13. IL4 exerts its effects through the activation of STAT6, a transcription factor involved in immune regulation. The gene encoding IL4 is located on chromosome 5q, clustered with genes encoding other cytokines, including IL3, IL5, IL13, and CSF2. These cytokines share regulatory elements, leading to coordinated expression. IL4 exists in two alternatively spliced isoforms, both of which contribute to its diverse biological activities.
Description
Recombinant Human Interleukin-4, produced in yeast, is a single-chain glycoprotein with a molecular weight of approximately 14.9 kDa. It comprises 129 amino acids and undergoes glycosylation. The purification process employs proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein solution, at a concentration of 0.2µm, was filtered through a 0.2µm filter and then lyophilized in a 20mM sodium phosphate buffer with a pH of 6.0. No carrier protein was added during the process.
Solubility
To reconstitute the lyophilized Interleukin-4, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions if required.
Stability
Lyophilized Interleukin-4 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C for long-term storage. Once reconstituted, Interleukin-4 should be stored at 4°C and used within 2-7 days. For future use, store it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined using SDS-PAGE analysis and is found to be greater than 98%.
Biological Activity
The biological activity of Interleukin-4 is evaluated by its ability to induce dose-dependent proliferation of human TF-1 cells and CD23 expression. Typically, a concentration range of 0.1–10.0 ng/ml is effective for most in vitro applications, with an ED₅₀ value ranging from 0.05 to 0.4 ng/ml.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Source
Pichia pastoris.
Product Science Overview
Functions and Biological Roles
IL-4 has several biological roles, including:
- Stimulation of B and T cell proliferation: IL-4 promotes the growth and differentiation of B cells into plasma cells, which are responsible for antibody production .
- Class switching to IgE: IL-4 induces B cells to switch from producing other antibody classes to producing IgE, which is involved in allergic responses .
- Regulation of macrophages: IL-4 promotes the alternative activation of macrophages into M2 cells, which are involved in wound repair and fibrosis .
Recombinant Human IL-4
Production and Quality Control
Recombinant human IL-4 is produced in yeast and undergoes several quality control measures to ensure its purity and biological activity. The purity of the recombinant IL-4 is typically greater than 98%, as determined by high-performance liquid chromatography (HPLC) and SDS-PAGE analysis . The biological activity is confirmed using cell proliferation assays .
Applications in Research
Storage and Stability
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