IL 4 Human, HEK

Interleukin-4 Human Recombinant, HEK
Cat. No.
BT5210
Source
HEK.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as obsereved by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL-4 Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 14-19kDa due to glycosylation.
The IL-4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 4 (IL-4) is a cytokine with diverse roles in the immune system, primarily known for its role in regulating allergic responses and promoting antibody production. Produced by activated T cells, IL-4 binds to its specific receptor (IL-4R) on target cells, initiating signaling pathways that lead to various downstream effects. One of its key functions is the induction of immunoglobulin class switching to IgE, the antibody type involved in allergic reactions. IL-4 also plays a role in the differentiation of T helper 2 (Th2) cells, which are involved in humoral immunity and allergic inflammation. Additionally, IL-4 exhibits anti-inflammatory properties in certain contexts.
Description
This product consists of recombinant human IL-4, produced in HEK cells. It appears as a glycosylated monomer, with a molecular weight ranging from 14 to 19 kDa due to variations in glycosylation. The purification process employs proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The IL-4 protein was lyophilized from a 0.2µm filtered solution (0.68mg/ml) in 1xPBS.
Solubility
To reconstitute the lyophilized IL-4, it is recommended to dissolve it in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Stability
Lyophilized IL-4 remains stable for 3 weeks at room temperature but should be stored desiccated at temperatures below -18°C for optimal long-term preservation. Once reconstituted, IL-4 should be stored at 4°C and used within 2-7 days. For extended storage, adding a carrier protein like 0.1% HSA or BSA is recommended. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of this product was determined based on its ability to stimulate the proliferation of human TF-1 cells (a human erythroleukemic indicator cell line) in a dose-dependent manner. The activity was found to be 0.17 ng/ml.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Source
HEK.

Product Science Overview

Introduction

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in the regulation of immune responses. It is produced by various cell types, including mast cells, basophils, and a subset of activated T cells . IL-4 is a complex glycoprotein mapped to human chromosome 5q31.1 .

Structure and Production

IL-4 is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation . When expressed in human embryonic kidney (HEK) 293 cells, IL-4 retains its authentic glycosylation, which is absent when expressed in E. coli . This glycosylation contributes to the stability of IL-4 in cell growth media and other applications .

Biological Functions

IL-4 serves as a ligand for the interleukin-4 receptor (IL-4R) and interacts with interleukin-13 (IL-13) . It regulates the production of immunoglobulin E (IgE) and immunoglobulin G1 (IgG1) by B cells . Additionally, IL-4 favors glial and neuronal differentiation and regulates the differentiation of naive CD4+ T cells to T helper type 2 (TH2) cells .

Clinical Significance

Polymorphisms in the IL-4 gene are implicated in various immune and inflammatory disorders . Elevated levels of IL-4 have been observed in conditions such as cerebral infarction . The recombinant form of IL-4, expressed in HEK 293 cells, is used in various research and clinical applications due to its stability and biological activity .

Applications

Recombinant human IL-4 is widely used in cell culture applications to study its effects on immune cells and its role in various diseases . It is also used to induce its cognate signaling pathway in HEK-Blue™ IL-4/IL-13 cells .

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