IL 4 Human, His
Interleukin-4 Human Recombinant, His Tag
Cat. No.
BT5289
Source
Escherichia Coli.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-4 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 150 amino acids fragment (25-153) and having a total molecular mass of 17.2kDa.
The IL-4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The IL-4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-4, a pleiotropic cytokine primarily produced by activated T lymphocytes, basophils, and mast cells, exhibits diverse immune response-modulating functions across various cell types. It plays a crucial role in regulating isotype switching, inducing IgE production in B lymphocytes, and differentiating precursor T helper cells. IL-4 binds to both membrane-bound and soluble forms of the IL-4 receptor.
Description
Recombinant human Interleukin-4, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 150 amino acids (fragment 25-153) with a molecular weight of 17.2 kDa. The IL-4 protein includes a 20 amino acid His-tag at the N-terminus and undergoes purification via proprietary chromatographic methods.
Physical Appearance
A clear, sterile, and filtered solution.
Formulation
Interleukin-4 His-Tag is supplied in a buffer containing 20mM Tris-HCl and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Biological Activity
The ED50, representing the concentration at which 50% of the maximal effect is observed, is less than 0.5 ng/ml as determined by a cell proliferation assay using TF1 human erythroleukemic cells.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.
Product Science Overview
Structure and Production
Recombinant human Interleukin-4 (IL-4) is typically produced in Escherichia coli (E. coli) and is available as a lyophilized powder. The recombinant form of IL-4 is a 14.9 kDa protein containing 129 amino acid residues . The His tag, a sequence of histidine residues, is often added to the recombinant protein to facilitate purification and detection .
Biological Functions
IL-4 has several important biological functions, including:
- Regulation of Immune Responses: IL-4 is a key regulator of immune responses, particularly in the context of allergic inflammation and asthma .
- B Cell Activation: It stimulates B cell proliferation and differentiation, leading to the production of immunoglobulins such as IgE and IgG1 .
- T Cell Differentiation: IL-4 promotes the differentiation of naive T cells into TH2 cells, which are essential for the immune response against extracellular pathogens .
Applications
Recombinant human IL-4 is widely used in research and clinical applications, including:
- Cell Culture: It is used to study the effects of IL-4 on various cell types, including T cells and B cells .
- Immunotherapy: IL-4 is being investigated for its potential use in immunotherapy for allergic diseases and asthma .
- Angiogenesis Inhibition: IL-4 has been shown to inhibit VEGF-induced and βFGF-induced angiogenesis, making it a potential therapeutic target for cancer treatment .
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