IL 1 Beta Antibody, Biotin

Interleukin-1b, Mouse Anti-Human Biotin
Cat. No.
BT6370
Source
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Interleukin-1 beta (IL-1β) is a cytokine produced by activated macrophages that plays a crucial role in the inflammatory response. IL-1β stimulates thymocyte proliferation by inducing IL-2 release, promotes B-cell maturation and proliferation, and enhances fibroblast growth factor activity. It is recognized as an endogenous pyrogen and has been shown to stimulate the release of prostaglandin and collagenase from synovial cells.
Formulation
The antibody is supplied as a solution with a concentration of 1 mg/ml in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, the lyophilized antibody should be kept at 4°C in a dry environment. After reconstitution, if not intended for immediate use, it is recommended to aliquot the antibody and store it at -20°C.
Titer
For intracellular staining, use 10 µl of antibody per 1,000,000 cells. For direct ELISA, a 1:10,000 dilution will yield an optical density (O.D.) greater than 1.0 using alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories).
Solubility
To reconstitute the antibody, add sterile water (H2O). Mix gently, ensuring the sides of the vial are washed, and allow 30-60 seconds for complete dissolution before use.
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Purification Method
Ion Exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYR-hIL1b.
Immunogen
r.Human IL-1b.
Ig Subclass
Mouse IgG2b.

Product Science Overview

Background of Mouse Anti-Human Biotin

Mouse Anti-Human Biotin antibodies are monoclonal antibodies developed in mice that are specific to human antigens and conjugated with biotin . Biotin, also known as Vitamin B7, is a ubiquitous co-factor with many properties that make it extremely useful for molecular biology applications .

Applications and Uses

Mouse Anti-Human Biotin antibodies are widely used in various scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA, and Flow Cytometry . These antibodies can detect human IgG1 and other immunoglobulin isotypes, making them valuable tools for research and diagnostic purposes .

The antibodies are typically purified from tissue culture supernatant or ascites by affinity chromatography and conjugated with biotin under optimal conditions . They are used in sandwich ELISA assays, where biotin-conjugated antibodies serve as detecting antibodies in combination with capture antibodies .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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