IL 1 Beta Antibody
Description
Product Specs
Product Science Overview
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that plays a crucial role in the body’s immune response. It is produced primarily by activated monocytes and macrophages. IL-1β is involved in various cellular activities, including cell proliferation, differentiation, and apoptosis. It is a key mediator in the inflammatory response and is implicated in a variety of diseases, including autoimmune disorders, infectious diseases, and cancer.
IL-1β is synthesized as a 31 kDa precursor protein. To become active, this precursor must be cleaved by caspase-1 between Asp116 and Ala117, resulting in a 17 kDa mature form . The detection of this mature form is often used as an indicator of caspase-1 activity.
Mouse anti-human IL-1β antibodies are monoclonal antibodies developed in mice that specifically target human IL-1β. These antibodies are widely used in research and clinical applications to study the role of IL-1β in various diseases and to develop therapeutic strategies.
Applications
Mouse anti-human IL-1β antibodies can be used in several scientific applications, including:
- Western Blotting (WB): To detect IL-1β protein levels in cell or tissue extracts.
- Enzyme-Linked Immunosorbent Assay (ELISA): To quantify IL-1β levels in biological samples.
- Immunohistochemistry (IHC): To visualize IL-1β expression in tissue sections.
- Immunocytochemistry (ICC): To detect IL-1β in cultured cells.
- Flow Cytometry: To analyze IL-1β expression on the cell surface or within cells .
Specificity and Cross-Reactivity
Mouse anti-human IL-1β antibodies are designed to specifically bind to human IL-1β. They do not cross-react with IL-1β from other species, such as mouse, rat, rabbit, or macaques . This specificity is crucial for accurate detection and quantification of human IL-1β in various experimental settings.
Types of Mouse Anti-Human IL-1β Antibodies
There are different types of mouse anti-human IL-1β antibodies available, including:
- Monoclonal Antibodies: These are produced by a single clone of B cells and are highly specific to a single epitope on the IL-1β molecule.
- Polyclonal Antibodies: These are produced by multiple clones of B cells and can recognize multiple epitopes on the IL-1β molecule.
- Recombinant Monoclonal Antibodies: These are genetically engineered antibodies that combine the specificity of monoclonal antibodies with the consistency and reproducibility of recombinant technology .
