IL 1 beta Monkey
Description
Product Specs
Interleukin-1 beta (IL-1β) is a crucial signaling molecule in the immune system, primarily produced by activated macrophages. It plays a vital role in stimulating the proliferation of thymocytes by triggering the release of interleukin-2 (IL-2). Additionally, IL-1β contributes to the maturation and proliferation of B cells and regulates fibroblast growth factor activity. As an endogenous pyrogen, IL-1β is involved in inflammatory responses and is known to induce prostaglandin release from synovial cells.
Product Science Overview
The recombinant form of IL-1β from rhesus macaques is typically produced using Escherichia coli (E. coli) expression systems . The mature form of rhesus IL-1β consists of amino acids 117 to 269, with a molecular weight of approximately 17.3 kDa . This recombinant protein shares 96% amino acid sequence identity with human IL-1β, making it a valuable tool for research purposes .
IL-1β is a secreted cytokine that has a broad range of biological activities. It stimulates thymocyte proliferation by inducing IL-2 release, promotes B-cell maturation and proliferation, and exhibits mitogenic fibroblast growth factor (FGF)-like activity . Additionally, IL-1β can stimulate the release of prostaglandin and collagenase from synovial cells . These activities make IL-1β a key player in inflammatory responses and immune regulation.
Recombinant IL-1β from rhesus macaques is used extensively in research to study its role in various diseases and immune responses. It is particularly valuable in studies involving multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), the animal model for MS . The cytokine’s ability to induce inflammation and modulate immune responses makes it a critical component in understanding the pathogenesis of these diseases.
The recombinant IL-1β protein is typically lyophilized from a filtered solution in phosphate-buffered saline (PBS) and can be reconstituted in sterile PBS containing bovine serum albumin (BSA) to enhance stability . It is recommended to store the protein at -20°C to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .
