IL 1 beta Equine
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
Product Specs
Product Science Overview
Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine that plays a crucial role in the regulation of immune responses, inflammation, and hematopoiesis. It is produced by various cell types, including activated macrophages, neutrophils, epithelial cells, and endothelial cells. The recombinant form of equine IL-1β is used extensively in research to study its effects and potential therapeutic applications in horses.
Recombinant equine IL-1β is typically produced in Escherichia coli (E. coli) cells. The protein is a non-glycosylated, homodimeric molecule consisting of 153 amino acids and has a molecular mass of approximately 17.3 kDa . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and activity.
IL-1β is a key mediator of the inflammatory response. It binds to the IL-1 receptor type I (IL-1RI) on target cells, which then associates with the IL-1 receptor accessory protein (IL-1RAcP) to form a high-affinity receptor complex capable of signal transduction . This interaction triggers a cascade of intracellular signaling pathways, leading to the activation of nuclear factor-kappa B (NF-κB) and the production of other pro-inflammatory cytokines.
Recombinant equine IL-1β is used in various research applications, including:
- Cell Proliferation Assays: It is used to measure the proliferation of immune cells, such as T cells, in response to IL-1β stimulation .
- Gene Expression Studies: Researchers use IL-1β to study its effects on gene expression in equine cells, particularly tenocytes, which are involved in tendon repair and regeneration .
- Inflammation Models: IL-1β is used to create in vitro models of inflammation to study the molecular mechanisms underlying inflammatory diseases and to test potential anti-inflammatory drugs.
Recombinant equine IL-1β is typically supplied as a lyophilized powder and should be reconstituted in sterile phosphate-buffered saline (PBS) containing at least 0.1% human or bovine serum albumin . The reconstituted protein should be stored at -20°C to -70°C to maintain its stability and activity. It is important to avoid repeated freeze-thaw cycles to prevent degradation of the protein.
