IL 1 Beta Antibody, FITC

Interleukin-1b, Mouse Anti-Human FITC
Cat. No.
BT6443
Source
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Upon activation, macrophages produce Interleukin-1 beta (IL-1β), a cytokine that plays a crucial role in stimulating thymocyte proliferation. This effect is mediated by the induction of IL-2 release, which in turn promotes the maturation and proliferation of B cells. Additionally, IL-1β contributes to fibroblast growth factor activity. As endogenous pyrogens, IL1B proteins are integral to the inflammatory response and have been shown to stimulate the release of prostaglandin and collagenase from synovial cells.
Formulation
The antibody is provided at a concentration of 1 mg/ml in a phosphate-buffered saline (PBS) solution after reconstitution.
Shipping Conditions
To ensure stability during transportation, the antibody is lyophilized and shipped at ambient temperature.
Storage Procedures
For long-term storage, it is recommended to keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, the antibody should be aliquoted and stored at -20°C if not intended for use within one month.
Titer
For intracellular staining procedures, it is recommended to use 10 µl of the antibody per 1,000,000 cells. When performing direct ELISA, a 1:10,000 dilution of the antibody will yield an optical density (O.D.) greater than 1.0 when using an alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories).
Solubility
To reconstitute the antibody, sterile water (H2O) should be used. Gently mix the solution, ensuring that the sides of the vial are washed. Allow 30-60 seconds for complete reconstitution before use.
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Purification Method
Ion Exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYR-hIL1b.
Immunogen
r.Human IL-1b.
Ig Subclass
Mouse IgG2b.

Product Science Overview

Mouse Anti-Human FITC

Mouse Anti-Human FITC refers to a monoclonal antibody produced in mice that is conjugated with fluorescein isothiocyanate (FITC), a fluorescent dye. This antibody is used in various immunological assays, including flow cytometry, immunohistochemistry, and immunofluorescence .

Characteristics and Applications

The monoclonal antibody is purified from tissue culture supernatant or ascites by affinity chromatography and is conjugated with FITC under optimal conditions . It is used to detect human immunoglobulins, such as IgG, in various biological samples . The antibody is typically stored undiluted at 4°C and protected from prolonged exposure to light .

Usage in Research

Mouse Anti-Human FITC antibodies are widely used in research to study the expression of specific proteins on the surface of cells. For example, the FITC anti-mouse/human CD11b antibody is used to study the expression of CD11b, a glycoprotein involved in cell-cell interactions . Similarly, the FITC Mouse Anti-Human IgG antibody is used to detect human IgG subclasses in various immunological assays .

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