HSA, Pichia Pastoris
Yellowish gel like solution.
Greater than 97% as determined by HPLC.
Description
The recombinant Albumin is purified by proprietary chromatographic techniques.
Product Specs
HSA, often derived from donors, is vital for maintaining blood volume. However, concerns about potential contamination, such as HIV and Hepatitis, have sparked significant interest in producing a recombinant form of HSA. This bioengineered version is molecularly identical to the naturally occurring blood protein.
The purification process of this recombinant albumin involves specialized chromatographic methods.
The solution has a gel-like consistency and a yellowish hue.
Repeated freezing and thawing of the product should be avoided.
High-Performance Liquid Chromatography (HPLC) analysis confirms a purity exceeding 97%.
Product Science Overview
Traditionally, HSA is sourced from human plasma. However, this method carries the risk of contamination with viruses and other pathogens . Additionally, the demand for HSA is substantial, with approximately 500 tonnes required annually for biological applications . To address these challenges, researchers have explored recombinant routes to produce functional HSA.
Pichia pastoris (syn. Komagataella phaffii) is a methylotrophic yeast that has been successfully used as a host for the production of several therapeutic proteins, including granulocyte colony-stimulating factor, human growth hormone, interferon, interleukin-2, factor IX, subtilisin QK, and monoclonal antibodies . The production of stable extracellular recombinant HSA (rHSA) in Pichia pastoris has been achieved at nearly 1 g/L at the shake-flask level . This was accomplished using a three-copy HSA expression cassette prepared in vitro .
Pichia pastoris offers several advantages as an expression platform for recombinant proteins:
- High Yield: The production of rHSA in Pichia pastoris can reach high levels, with productivity of 300 mg/L/d at the bioreactor level .
- Reduced Contamination Risk: Since Pichia pastoris is a yeast, the risk of contamination with human pathogens is significantly reduced compared to plasma-derived HSA .
- Cost-Effective: The use of Pichia pastoris for recombinant protein production is cost-effective due to its ability to grow to high cell densities on simple and inexpensive media .
