HSA Recombinant, Plant
Greater than 98% as determined by SDS-PAGE.
Description
HSA Human Recombinant produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa. The optimum concentration for recombinant Albumin to be used in cell culture ranges between 0.5gr to 2gr per liter. The recombinant Albumin is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Human Serum Albumin (HSA) is a crucial protein in the human body, primarily responsible for maintaining oncotic pressure and transporting various substances, including hormones, fatty acids, and drugs. Traditionally, HSA is extracted from human blood plasma, but this method has limitations, including the risk of viral transmission and limited supply. To address these challenges, scientists have developed recombinant HSA (rHSA) using various expression systems, including plants.
The production of recombinant proteins in plants, also known as molecular farming, has emerged as a promising alternative to traditional methods. Plants offer several advantages, such as low production costs, scalability, and reduced risk of contamination with human pathogens. Among the various plant species used for recombinant protein production, tobacco, rice, and maize are the most common.
The process of producing rHSA in plants involves several steps:
- Gene Cloning: The gene encoding HSA is first cloned into a suitable expression vector. This vector is then introduced into the plant cells using techniques such as Agrobacterium-mediated transformation or biolistic particle delivery.
- Plant Transformation: The transformed plant cells are cultured to regenerate whole plants. These transgenic plants express the rHSA gene, leading to the production of the protein in their tissues.
- Protein Extraction and Purification: The rHSA is extracted from the plant tissues and purified using chromatographic techniques to ensure high purity and functionality.
- Safety: Plant-based systems eliminate the risk of viral contamination associated with blood-derived HSA.
- Cost-Effectiveness: The production costs are significantly lower compared to traditional methods, making it a more economical option.
- Scalability: Plants can be easily cultivated on a large scale, allowing for mass production of rHSA.
Recombinant HSA produced in plants has various applications, including:
- Medical Use: rHSA is used in therapeutic treatments, such as volume replacement therapy and drug delivery systems.
- Cell Culture: It is used as a supplement in cell culture media to support the growth and maintenance of cells.
- Research: rHSA serves as a valuable tool in biochemical and pharmaceutical research.
