HSA, Sf9

Serum Albumin Human, Sf9
Cat. No.
BT15300
Source
Sf9, Baculovirus cells.
Synonyms
Albumin, Cell Growth Inhibiting Protein 42, Growth-Inhibiting Protein 20, Albumin (32 AA), Albumin (AA 34), Serum Albumin, PRO0883, PRO0903, PRO1341, ANALBA, FDAH, HAS, ALB.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HSA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-609 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 597 amino acids and having a molecular mass of 68kDa.
HSA shows multiple bands between 50-70kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Albumin is a crucial protein synthesized in the liver. Its production begins as preproalbumin and undergoes several processing steps, including removal of an N-terminal peptide and cleavage in the Golgi vesicles, to become the secreted albumin found in blood serum. Constituting approximately half of serum protein content, albumin serves as a vital carrier for molecules like steroids, fatty acids, and thyroid hormones. Additionally, it plays a role in maintaining extracellular fluid volume stability. Genetic mutations affecting the albumin gene can lead to the production of abnormal protein variants. Structurally, albumin is a soluble, monomeric protein with a molecular weight of 65,000. The human albumin gene spans 16,961 nucleotides and consists of 15 exons distributed symmetrically across three domains, believed to have originated from the triplication of an ancestral domain. Human serum albumin (HSA) is extensively utilized for stabilizing blood volume, typically sourced from donors. However, concerns regarding potential contamination risks, such as HIV and Hepatitis, have fueled significant interest in recombinant HSA production, which yields a protein identical to its natural counterpart.
Description
Recombinant HSA, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain consisting of amino acids 19 to 609. It also includes a 6-amino acid Histidine tag fused at the C-terminus, resulting in a total of 597 amino acids and a molecular weight of 68 kDa. When analyzed by SDS-PAGE under reducing conditions, HSA displays multiple bands between 50-70 kDa. The protein undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The HSA protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the HSA solution can be stored at 4°C. For extended storage, it is recommended to store the solution frozen at -20°C. Adding a carrier protein like HSA or BSA at a concentration of 0.1% is advisable for long-term storage to maintain protein stability. It's important to avoid repeated cycles of freezing and thawing.
Purity
The purity of HSA is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Albumin, Cell Growth Inhibiting Protein 42, Growth-Inhibiting Protein 20, Albumin (32 AA), Albumin (AA 34), Serum Albumin, PRO0883, PRO0903, PRO1341, ANALBA, FDAH, HAS, ALB.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
RGVFRRDAHK SEVAHRFKDL GEENFKALVL IAFAQYLQQC PFEDHVKLVN EVTEFAKTCV ADESAENCDK SLHTLFGDKL CTVATLRETY GEMADCCAKQ EPERNECFLQ HKDDNPNLPR LVRPEVDVMC TAFHDNEETF LKKYLYEIAR RHPYFYAPEL LFFAKRYKAA FTECCQAADK AACLLPKLDE LRDEGKASSA KQRLKCASLQ KFGERAFKAW AVARLSQRFP KAEFAEVSKL VTDLTKVHTE CCHGDLLECA DDRADLAKYI CENQDSISSK LKECCEKPLL EKSHCIAEVE NDEMPADLPS LAADFVESKD VCKNYAEAKD VFLGMFLYEY ARRHPDYSVV LLLRLAKTYE TTLEKCCAAA DPHECYAKVF DEFKPLVEEP QNLIKQNCEL FEQLGEYKFQ NALLVRYTKK VPQVSTPTLV EVSRNLGKVG SKCCKHPEAK RMPCAEDYLS VVLNQLCVLH EKTPVSDRVT KCCTESLVNR RPCFSALEVD ETYVPKEFNA ETFTFHADIC TLSEKERQIK KQTALVELVK HKPKATKEQL KAVMDDFAAF VEKCCKADDK ETCFAEEGKK LVAASQAALG LHHHHHH

Product Science Overview

Introduction

Serum albumin is a crucial protein found in human blood plasma. It is the most abundant protein in human blood, constituting about half of the serum protein. Serum albumin is produced in the liver and plays a vital role in maintaining the colloidal osmotic pressure of blood and in the transportation of various substances, including hormones, fatty acids, and drugs .

Structure and Function

Human serum albumin (HSA) is a monomeric protein that is soluble in water. It has a molecular mass of approximately 68 kDa and consists of a single polypeptide chain. The protein is known for its ability to bind to a wide range of endogenous and exogenous substances, which makes it an essential carrier protein in the bloodstream .

The primary functions of serum albumin include:

  • Maintaining osmotic pressure: Serum albumin helps regulate the osmotic pressure of blood, which is crucial for the proper distribution of body fluids between blood vessels and tissues.
  • Transporting substances: It binds to various substances such as fatty acids, hormones, bilirubin, and drugs, facilitating their transport through the bloodstream.
  • Buffering pH: Serum albumin acts as a buffer, helping to maintain the pH balance of blood.
Serum Albumin Human, Sf9

Serum Albumin Human, Sf9, refers to human serum albumin produced using the Sf9 insect cell line. The Sf9 cell line is derived from the fall armyworm (Spodoptera frugiperda) and is commonly used in biotechnology for the production of recombinant proteins. The use of Sf9 cells allows for the production of glycosylated proteins, which can be important for the proper folding and function of certain proteins .

Production and Purification

The production of human serum albumin in Sf9 cells involves the use of recombinant DNA technology. The gene encoding human serum albumin is inserted into a baculovirus vector, which is then used to infect the Sf9 cells. The infected cells produce the recombinant human serum albumin, which can then be harvested and purified using various chromatographic techniques .

The recombinant human serum albumin produced in Sf9 cells is a single, glycosylated polypeptide chain consisting of 597 amino acids. It is fused to a 6 amino acid His Tag at the C-terminus, which aids in the purification process. The protein shows multiple bands between 50-70 kDa on SDS-PAGE under reducing conditions .

Applications

Human serum albumin produced in Sf9 cells has several applications in research and biotechnology:

  • Drug delivery: Due to its ability to bind to various substances, serum albumin is used in the development of advanced drug delivery systems. Albumin nanostructures can be used to improve the delivery and efficacy of therapeutic agents .
  • Cell culture: Serum albumin is often used in the formulation of media for the culture of mammalian cells. It provides essential nutrients and helps maintain the osmotic balance in the culture environment .
  • Diagnostic assays: Human serum albumin is used in various diagnostic assays to measure the levels of albumin in blood and urine, which can be indicative of liver and kidney function .

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