HSA Recombinant, HEK
Human Serum Albumin Recombinant, HEK
Cat. No.
BT15075
Source
HEK.
Synonyms
Serum albumin, ALB, PRO0883, PRO0903, PRO1341, DKFZp779N1935, GIG20, GIG42, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696, SA, HSA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as obsereved by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Recombinant Human Serum Albumin produced in HEK cells is a glycosylated monomer, having a molecular weight range of 60-65kDa due to glycosylation.
The HSA is purified by proprietary chromatographic techniques.
The HSA is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Albumin, a vital blood protein, is created in the liver through a multi-step process. Initially synthesized as preproalbumin, it undergoes cleavage events to become proalbumin and finally mature albumin. This soluble protein constitutes roughly half of blood serum protein content. Primarily, albumin acts as a transporter for molecules like steroids, fatty acids, and thyroid hormones. Moreover, it contributes to maintaining stable extracellular fluid volume. Genetic mutations affecting albumin production can lead to protein abnormalities. Structurally, albumin is a globular, non-glycosylated protein with a molecular weight of 65,000. The gene responsible for encoding human albumin spans 16,961 nucleotides and comprises 15 exons, organized symmetrically across three domains believed to have originated from a single ancestral domain. Due to concerns about contamination risks associated with human-derived albumin, there's growing interest in recombinant forms, which exhibit identical characteristics to the naturally occurring protein.
Description
Recombinant Human Serum Albumin (HSA), produced in HEK cells, is a monomeric protein with a molecular weight ranging from 60 to 65 kDa due to glycosylation. The purification process involves specialized chromatographic techniques.
Physical Appearance
White, sterile-filtered powder obtained through freeze-drying.
Formulation
The HSA protein was lyophilized from a 0.92mg/ml solution in 1xPBS.
Solubility
To reconstitute the lyophilized HSA, it is advised to use sterile 1xPBS.
Stability
While lyophilized HSA remains stable at room temperature for up to 3 weeks, it is recommended to store it in a dry state below -18°C. After reconstitution, HSA should be kept at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended, preferably with the addition of a carrier protein like 0.1% HSA or BSA. Avoid repeated freezing and thawing.
Purity
The purity is determined to be greater than 95% based on SDS-PAGE analysis.
Synonyms
Serum albumin, ALB, PRO0883, PRO0903, PRO1341, DKFZp779N1935, GIG20, GIG42, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696, SA, HSA.
Source
HEK.
Product Science Overview
Recombinant Human Serum Albumin (rHSA)
Recombinant Human Serum Albumin (rHSA) is a form of HSA produced through recombinant DNA technology. This method involves inserting the human albumin gene into host cells, such as yeast, bacteria, or mammalian cells, to produce HSA. The recombinant form is highly pure and free from potential contaminants and impurities that can be present in plasma-derived HSA .
Production in HEK Cells
HEK (Human Embryonic Kidney) 293 cells are commonly used for producing recombinant proteins, including rHSA. These cells are preferred due to their high transfection efficiency and ability to perform post-translational modifications similar to human cells . The rHSA produced in HEK cells is glycosylated, which means it has sugar molecules attached to it, resulting in a molecular weight range of 60-65 kDa .
Advantages of rHSA
- Purity and Safety: rHSA is free from the risk of contamination with blood-borne pathogens such as HIV and Hepatitis, which can be a concern with plasma-derived HSA .
- Consistency: The recombinant production process ensures a consistent and well-defined product, which is crucial for applications in pharmaceuticals and biotechnology .
- Scalability: Recombinant production allows for scalable manufacturing processes, making it easier to meet the demand for HSA in various applications .
Applications of rHSA
- Pharmaceuticals: rHSA is used as a stabilizer for vaccines and therapeutic proteins.
- Cell Culture: It is used in cell culture media to support the growth and maintenance of cells.
- Research: rHSA is utilized in various research applications, including drug delivery studies and protein binding assays.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
