gAcrp30 Human, His

The human recombinant globular adiponectin with a His tag is a truncated form of the full-length adiponectin protein. It consists of a 60 amino acid N-terminal collagenous region and a 137 amino acid C-terminal C1q-like globular domain. The His tag is a sequence of histidine residues added to the N-terminal of the protein to facilitate purification and detection .

The recombinant globular adiponectin is typically produced in Escherichia coli (E. coli) and purified to a high degree of purity, often greater than 95% as determined by SDS-PAGE. The protein is lyophilized from a PBS buffer and can be reconstituted with deionized water .

Adiponectin is known for its wide-ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity. The secretion of adiponectin is stimulated by insulin and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In metabolic contexts, adiponectin acts as an anti-inflammatory agent, but it can exert pro-inflammatory effects in non-metabolic disorders such as rheumatoid arthritis and inflammatory bowel disease .

Adiponectin interacts with several receptors, including AdipoR1, AdipoR2, calreticulin, and Cadherin-13/T-Cadherin. These interactions are crucial for its role in various signaling pathways that regulate metabolic processes .

Recombinant globular adiponectin is widely used in research to study its effects on metabolism, inflammation, and related diseases. It is also used in assays to measure its activity and interactions with other proteins and receptors.

For long-term storage, the lyophilized protein should be kept at -20°C. After reconstitution, it can be stored at 4°C for a limited period, typically up to two weeks. The stability of the protein is maintained for up to 24 months when stored properly .