Acrp30 Human, HEK
Adiponectin Human Recombinant, HEK
Cat. No.
BT6235
Source
HEK293 (Human Embryonic Kidney cell line).
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
White lyophilized (freeze-dried) powder.
Purity
Greater than 98% as determined by HPLC and SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
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Description
The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.
Product Specs
Introduction
Adiponectin, a 244-amino acid protein encoded by the apM1 gene, is primarily produced by adipose cells (hence, an adipokine). Structurally, it belongs to the soluble defense collagen superfamily, featuring a collagen-like domain similar to collagen VIII, X, and complement factor C1q-like globular domain. Adiponectin monomers assemble into homotrimers, serving as the foundation for higher-order complexes found circulating in serum.
Description
Recombinant human Acrp30, expressed in HEK293 cells, is engineered with a FLAG tag.
Biological Activity
The ability of mammalian cell-derived human Adiponectin to suppress glucose production was assessed using an in vitro gluconeogenesis assay with primary hepatocytes. The half-maximal effective concentration (ED50) was determined to be approximately 6 micrograms per milliliter.
Physical Appearance
Lyophilized powder, white in color.
Formulation
Prior to lyophilization, the product undergoes filtration (0.4 micrometers) and is prepared in a 50mM phosphate buffer containing 75mM NaCl at pH 7.4, with a concentration of 0.5 milligrams per milliliter.
Solubility
To generate a working stock solution of roughly 0.5 milligrams per milliliter, reconstitute the lyophilized pellet with deionized water, ensuring complete dissolution. This product is not sterile; for cell culture applications, it requires filtration using an appropriate sterile filter.
Stability
For long-term storage, keep lyophilized AdipoQ at -20 degrees Celsius. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. Reconstituted protein exhibits stability at 4 degrees Celsius for a limited duration, showing no noticeable changes for up to two weeks. The lyophilized protein remains stable for 24 months when stored at -20 degrees Celsius.
Purity
High-performance liquid chromatography (HPLC) and SDS-PAGE analysis confirm a purity exceeding 98%.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
HEK293 (Human Embryonic Kidney cell line).
Amino Acid Sequence
ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.
Product Science Overview
Structure and Function
Adiponectin is composed of a carboxyl-terminal globular domain and an amino-terminal collagen domain . It circulates in the plasma in various multimeric forms, including trimers, hexamers, and high molecular weight (HMW) complexes. The HMW form is considered the most biologically active, particularly in mediating insulin-sensitizing effects .
Production in HEK 293 Cells
Human recombinant adiponectin is often produced using HEK 293 cells, a human embryonic kidney cell line. This system is preferred because it ensures proper post-translational modifications, such as glycosylation, which are essential for the protein’s stability and function . The recombinant protein is typically expressed with a 6-His tag at the C-terminus to facilitate purification .
Biological Actions
Adiponectin exerts its effects through several mechanisms:
- Insulin Sensitivity: It enhances insulin sensitivity by increasing glucose uptake and fatty acid oxidation in skeletal muscle and liver tissues.
- Anti-inflammatory Effects: Adiponectin inhibits the expression of pro-inflammatory cytokines and adhesion molecules in endothelial cells, reducing inflammation and preventing atherosclerosis .
- Lipid Metabolism: It promotes lipid catabolism and reduces lipid accumulation in tissues, thereby protecting against obesity-related complications .
Clinical Significance
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