Acrp30 Human, HMW

Adiponectin glycosylated Human Recombinant, HMW Rich
Cat. No.
BT6377
Source
HEK293.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Adiponectin Human Recombinant HMW Rich produced in HEK cells is a single, glycosylated, polypeptide chain (19-244) containing a total of 226 amino acids, having a molecular mass of 24.6kDa (calculated). Human Acrp30 HMW Rich migrates on SDS-PAGE under non-reducing conditions at ~ 884 kDa.

Product Specs

Introduction
Adiponectin, a 244-amino acid protein encoded by the apM1 gene, is primarily produced by adipose cells (hence, an Adipokine). This protein plays a crucial role in various physiological processes. Structurally, adiponectin belongs to the soluble defense collagen superfamily, characterized by a collagen-like domain similar to collagen VIII, X, and a globular domain resembling complement factor C1q. The basic unit of adiponectin is a homotrimer, which can assemble into higher-order complexes found circulating in serum.
Description

Recombinant Human Adiponectin (HMW Rich), expressed in HEK cells, is a single-chain polypeptide comprising amino acids 19-244. This glycosylated protein has a molecular weight of 24.6 kDa (calculated) and consists of 226 amino acids. Under non-reducing conditions on SDS-PAGE, it migrates at approximately 884 kDa.

Physical Appearance
White powder, lyophilized (freeze-dried) and sterile-filtered.
Formulation
Acrp30 was sterile-filtered (0.4µm) and lyophilized at a concentration of 0.5mg/ml in a buffer of 20mM Tris, 50mM NaCl, pH 7.5, and 1mM CaCl2.
Solubility
To prepare a working solution, add deionized water to the lyophilized pellet to achieve a concentration of 0.5mg/ml. Allow the pellet to dissolve completely. For cell culture applications, ensure sterility by filtering the reconstituted protein through a suitable sterile filter before use.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein and store at 4°C to minimize repeated freeze-thaw cycles. The reconstituted protein is stable at 4°C for a limited period.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
HEK293.
Amino Acid Sequence
ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.

Product Science Overview

Structure and Forms

Adiponectin is a 244 amino acid protein that belongs to the soluble defense collagen superfamily. It has a collagen-like domain structurally homologous with collagen VIII and X and a complement factor C1q-like globular domain . The protein forms homotrimers, which are the building blocks for higher-order complexes found circulating in serum .

There are three main forms of adiponectin:

  1. Low Molecular Weight (LMW)
  2. Medium Molecular Weight (MMW)
  3. High Molecular Weight (HMW)

The HMW form is considered the most biologically active and is particularly important in mediating the insulin-sensitizing effects of adiponectin .

Human Recombinant Adiponectin (HMW Rich)

Human recombinant adiponectin (HMW rich) is produced in HEK293 cells and is a single, glycosylated polypeptide chain containing 226 amino acids, with a molecular mass of 24.6 kDa (calculated) . This recombinant form is designed to mimic the naturally occurring HMW adiponectin, making it valuable for research and therapeutic applications.

Physical Appearance and Formulation:

  • Filtered white lyophilized (freeze-dried) powder .
  • Formulated in 20mM Tris, 50mM NaCl, pH 7.5, and 1mM CaCl2 .

Solubility and Stability:

  • Recommended to dissolve in deionized water to a working concentration of 0.5mg/ml .
  • Store lyophilized protein at -20°C and avoid repeated freezing/thawing cycles .
Biological Functions

Adiponectin is involved in various physiological processes, including:

  • Regulation of glucose levels: Enhances insulin sensitivity and glucose utilization.
  • Fatty acid oxidation: Promotes the breakdown of fatty acids.
  • Anti-inflammatory effects: Inhibits the expression of pro-inflammatory cytokines like TNF-alpha .
  • Cardiovascular protection: Plays a role in reducing the risk of atherosclerosis and other cardiovascular diseases .
Applications in Research and Medicine

Due to its significant role in metabolic processes, adiponectin is a subject of extensive research. It is used in studies related to:

  • Diabetes: Understanding its role in insulin sensitivity and potential therapeutic applications.
  • Obesity: Investigating its impact on fat metabolism and weight management.
  • Cardiovascular diseases: Exploring its protective effects against heart diseases .

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