Acrp30 Antibody
Description
Product Specs
Product Science Overview
Adiponectin, also known as ACRP30, apM-1, and ADIPOQ, is a protein hormone produced and secreted by adipose tissue. It plays a crucial role in regulating glucose levels and fatty acid breakdown. Adiponectin is involved in various metabolic processes, including:
- Insulin Sensitization: Adiponectin enhances insulin sensitivity, making it a key player in glucose homeostasis. It suppresses glucose production in the liver and promotes fatty acid oxidation in skeletal muscle .
- Anti-inflammatory Effects: Adiponectin has anti-inflammatory properties, reducing the production of pro-inflammatory cytokines and increasing anti-inflammatory cytokines like IL-10 .
- Anti-atherogenic Effects: It helps prevent the formation of atherosclerotic plaques, thus protecting against cardiovascular diseases .
Mouse anti-human adiponectin antibodies are monoclonal antibodies developed in mice that specifically target human adiponectin. These antibodies are widely used in research and diagnostic applications due to their high specificity and affinity for human adiponectin.
Applications
Mouse anti-human adiponectin antibodies are utilized in various scientific applications, including:
- Western Blot (WB): Used to detect adiponectin in protein samples.
- Immunohistochemistry (IHC): Helps visualize adiponectin in tissue sections.
- Immunocytochemistry (ICC): Used to detect adiponectin in cultured cells.
- Enzyme-Linked Immunosorbent Assay (ELISA): Quantifies adiponectin levels in biological samples.
- Flow Cytometry: Analyzes adiponectin expression in cell populations .
Production and Characteristics
These antibodies are produced by immunizing mice with human adiponectin or its recombinant forms. The spleen cells from the immunized mice are fused with myeloma cells to create hybridomas, which are then screened for the production of specific antibodies. The selected hybridomas are cultured, and the antibodies are purified from the culture supernatant.
Mouse anti-human adiponectin antibodies are available in various forms, including:
- Monoclonal Antibodies: Produced by a single clone of hybridoma cells, offering high specificity and consistency.
- Polyclonal Antibodies: Produced by multiple clones, recognizing different epitopes on the adiponectin molecule.
- Recombinant Monoclonal Antibodies: Engineered for enhanced performance and reproducibility .
Adiponectin and its antibodies have significant implications in research and clinical settings:
- Diabetes Research: Given its role in insulin sensitization, adiponectin is a potential therapeutic target for type 2 diabetes .
- Obesity Studies: Adiponectin levels are inversely correlated with body fat percentage, making it a marker for obesity-related studies .
- Cardiovascular Research: Its anti-atherogenic properties make adiponectin a focus of cardiovascular disease research .
- Inflammatory Diseases: The anti-inflammatory effects of adiponectin are explored in various inflammatory conditions .
