gAcrp30 Mouse
Globular Adiponectin Mouse Recombinant
Cat. No.
BT8067
Source
Escherichia Coli.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The globular domain of Adiponectin Mouse Recombinant / Acrp30 Mouse contains 138 amino acid residues from a.a. 111-247 having molecular mass of 16 kDa was over expressed in E.coli and purified by using conventional chromatography techniques.
Product Specs
Introduction
Adiponectin, a 30 kDa adipocyte complement-related protein comprising 247 amino acids, is exclusively expressed in differentiated adipocytes. APM-1 (Acrp30 Mouse), a member of the complement factor C1q family, consists of a signal sequence, a non-homologous sequence, a collagen domain, and a globular domain (gAcrp30). Adiponectin expression is reduced in various obese and insulin-resistant states in humans, monkeys, and mice. Injecting Acrp30 Mouse (247aa) or gAcrp30 lowers serum glucose and free fatty acid levels in mice.
Description
The recombinant globular domain of mouse Adiponectin/Acrp30, encompassing amino acid residues 111-247 with a molecular mass of 16 kDa, was overexpressed in E. coli and purified using standard chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
Acrp30 Mouse solution (1 mg/ml) in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
Escherichia Coli.
Amino Acid Sequence
MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITVYMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYADNVNDSTFTG FLLYHDTN
Product Science Overview
Structure and Expression
Adiponectin, also known as Acrp30, is a member of the complement factor C1q family. It consists of several domains:
- Signal Sequence: Directs the protein to its proper location in the cell.
- Non-homologous Sequence: Unique to adiponectin.
- Collagen Domain: Involved in the formation of higher-order structures.
- Globular Domain: The focus of this article, known for its potent biological effects .
Recombinant globular adiponectin is typically expressed in Escherichia coli to ensure high purity and biological activity. The recombinant form is often used in research to study its effects on various metabolic processes .
Biological Functions
Globular adiponectin has several key functions:
- Metabolic Regulation: It enhances glucose utilization and fatty acid oxidation, making it a critical player in maintaining energy homeostasis .
- Anti-inflammatory Effects: It antagonizes TNF-alpha, a pro-inflammatory cytokine, thereby reducing inflammation in tissues such as the liver and macrophages .
- Insulin Sensitivity: By stimulating AMPK phosphorylation in the liver and skeletal muscle, it improves insulin sensitivity, which is beneficial for managing diabetes .
Research Applications
Due to its significant role in metabolism and inflammation, globular adiponectin is widely used in research:
- Diabetes Research: Its ability to enhance insulin sensitivity makes it a valuable tool for studying diabetes and potential treatments .
- Obesity Studies: Given its role in fat metabolism, it is often used to understand obesity and related metabolic disorders .
- Cardiovascular Research: Its anti-inflammatory properties are explored for potential benefits in cardiovascular diseases .
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