Acrp30 Human, His
Adiponectin Human Recombinant, His tag
Cat. No.
BT6313
Source
Escherichia Coli.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Acrp30 Human purity is greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
The Acrp30 Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 Human and 12 additional amino acid residues - HisTag (underlined).
Product Specs
Introduction
Adiponectin, also known as Acrp30, AdipoQ, and GBP-28, is a 244-amino acid protein encoded by the apM1 gene. This protein plays an active physiological role and is primarily produced by adipose cells (making it an adipokine). Structurally, adiponectin belongs to the soluble defense collagen superfamily, possessing a collagen-like domain similar to collagen VIII and X, and a globular domain resembling complement factor C1q. APM-1 forms homotrimers, serving as the foundation for larger complexes found circulating in serum.
Description
This product consists of recombinant human Acrp30 with an N-terminal His tag. Produced in E. coli, the His-tagged Acrp30 fusion protein has a molecular weight of 26.4 kDa. It comprises 230 amino acid residues of the human Acrp30 protein and an additional 12 amino acid residues forming the His tag.
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
This Acrp30 Human product undergoes filtration (0.4 μm) and lyophilization from a solution of 0.5 mg/ml in 0.02M Tris buffer (pH 7.5) and 0.15M NaCl.
Solubility
To create a working solution, add deionized water to the lyophilized pellet, aiming for a concentration of about 0.5 mg/ml. Allow the pellet to dissolve entirely. Please note that this product is not sterile. Before use in cell culture, sterilize the product by filtration using an appropriate sterile filter.
Stability
Store lyophilized Acrp30 Human at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. Reconstituted Acrp30 Human remains stable at 4°C for a limited time, showing no noticeable changes for up to two weeks.
Purity
The purity of Acrp30 Human exceeds 95%, as determined by SDS-PAGE analysis.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHHGSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN.
Product Science Overview
Functions of Adiponectin
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Regulation of Glucose and Lipid Metabolism:
- Adiponectin enhances insulin sensitivity, helping to regulate blood sugar levels.
- It promotes fatty acid oxidation, which can help reduce fat accumulation.
-
Anti-Inflammatory Effects:
- Adiponectin has anti-inflammatory properties that can help mitigate chronic inflammation.
-
Cardiovascular Health:
- It can influence vascular health and has been associated with a lower risk of cardiovascular diseases.
Levels and Health Implications
- Normal Levels: Generally, higher levels of adiponectin are associated with a lower risk of metabolic syndrome, type 2 diabetes, and cardiovascular diseases.
- Low Levels: Reduced adiponectin levels are often linked with obesity, insulin resistance, and other metabolic disorders.
Factors Influencing Adiponectin Levels
- Body Weight: Higher body fat, especially visceral fat, can lower adiponectin levels.
- Diet and Exercise: Regular physical activity and a healthy diet can increase adiponectin levels.
- Genetics: Genetic factors can also influence individual adiponectin levels.
Recombinant Adiponectin (Human, His tag)
The recombinant form of adiponectin is created as a protein with a polyhistidine (His) tag, which facilitates its purification and detection in laboratory settings. Here are some key details:
- Production: The recombinant adiponectin is typically produced in Escherichia coli (E. coli) or other expression systems.
- Structure: It is a 24 kDa protein containing 173 amino acid residues of the human adiponectin sequence.
- Purity: The recombinant protein is usually purified to greater than 90% purity as determined by SDS-PAGE.
- Formulation: It is often lyophilized (freeze-dried) and can be reconstituted in a suitable buffer for experimental use.
Applications in Research
Recombinant adiponectin is used in various research applications, including:
- Studying Metabolic Disorders: Understanding the role of adiponectin in conditions like obesity, diabetes, and cardiovascular diseases.
- Drug Development: Exploring potential therapeutic uses of adiponectin or its analogs.
- Cell Signaling Studies: Investigating the signaling pathways influenced by adiponectin and its receptors.
In summary, adiponectin is a vital hormone with significant roles in metabolism and health. The recombinant form, tagged with a His tag, is an essential tool in scientific research, helping to unravel the complexities of this important protein .
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