Acrp30 Porcine, HEK

Adiponectin Porcine Recombinant, HEK derived
Cat. No.
BT7547
Source
HEK293 (Human Embryonic Kidney cell line).
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
Purity
Greater than 90% as determined by SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The Acrp30 Porcine Recombinant contains a total of 238 amino acids having a molecular Mass of 26kDa. The Porcine Adiponectin is fused to a 13 amino acid long N-terminal FLAG tag.

Product Specs

Introduction
Adiponectin, encoded by the apM1 gene, is a 244-amino acid protein primarily produced by adipose cells (making it an adipokine). This protein plays a significant role in physiological processes. Structurally, adiponectin belongs to the soluble defense collagen superfamily. It possesses a collagen-like domain with structural similarities to collagen VIII, collagen X, and the globular domain of complement factor C1q. APM-1 forms homotrimers, which serve as the foundation for higher-order complexes found circulating in serum.
Description
Recombinant Porcine Acrp30 is a 238-amino acid protein with a molecular weight of 26kDa. A 13-amino acid N-terminal FLAG tag is fused to this Porcine Adiponectin.
Formulation
Lyophilized from a 0.5mg/ml solution in 20mM Tris buffer with 50mM NaCl at pH 7.5. The solution is sterile filtered before lyophilization.
Solubility
Reconstitute the lyophilized pellet by adding deionized water and allow for complete dissolution.
Stability
Store lyophilized AdipoQ at -20°C for long-term storage. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period; no changes are observed after two weeks at 4°C. The lyophilized protein is stable for 24 months when stored at -20°C.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
HEK293 (Human Embryonic Kidney cell line).
Amino Acid Sequence
HVDYKDDDDK PAGETTEKPG ALLPMPKGAC AGWMAGIPGH PGHNGTPGRD GRDGVPGEKG EKGDTGLTGP KGDTGESGVT GVEGPRGFPG IPGRKGEPGE SAYVYRSAFS VGLETRVTVP NMPIRFTKIF YNQQNHYDVT TGKFHCNIPG LYYFSFHITV LKDVKVSLYK DKAVLFTYDQ QDKNVDQASG VLLYLEKGDQ WLQAYGDEEN GVYADNVNDS FTGFLLYHNIE.

Product Science Overview

Introduction

Adiponectin is a hormone secreted by adipocytes (fat cells) that plays a crucial role in regulating glucose levels and fatty acid breakdown. It exists in various multimeric forms, with the high molecular weight (HMW) form being the most biologically active. Adiponectin has significant implications in metabolic processes, including insulin sensitivity and anti-inflammatory responses .

Recombinant Adiponectin Production

Recombinant adiponectin is produced using various expression systems, including Escherichia coli, mammalian cells, and insect cells. Among these, the human embryonic kidney (HEK) 293 cell line is a popular choice due to its ability to produce properly folded and post-translationally modified proteins .

Porcine Adiponectin

Porcine adiponectin shares a high degree of similarity with human adiponectin, making it a valuable model for studying adiponectin’s functions and therapeutic potential. Recombinant porcine adiponectin can be produced in HEK 293 cells, ensuring the protein is correctly folded and functional .

HEK Derived Adiponectin

HEK 293 cells are widely used in biotechnology for producing recombinant proteins. These cells are advantageous because they can perform complex post-translational modifications, such as glycosylation, which are essential for the biological activity of many proteins, including adiponectin .

Applications and Benefits

Recombinant adiponectin, particularly the HMW form, has shown promise in various therapeutic applications. It has been studied for its potential to treat metabolic disorders, such as diabetes and obesity, due to its insulin-sensitizing and anti-inflammatory properties . Additionally, recombinant adiponectin can be used in research to better understand its role in metabolic and cardiovascular diseases .

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