gAcrp30 Human
Greater than 95% as determined by SDS-PAGE.
Description
gAcrp30 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 145 amino acids and having a molecular mass of 16.7kDa.
The gAcrp30 is purified by proprietary chromatographic techniques.
Product Specs
The globular protein gAcrp30 is a product of adiponectin's proteolytic processing. Adiponectin, a highly abundant plasma protein constituting up to 0.05% of total serum protein, is exclusively produced and secreted by adipocytes. Sharing similarities with Adiponectin, gAcrp30 possesses the ability to reduce hyperglycemia and counteract insulin resistance. Moreover, gAcrp30 plays a crucial role in promoting fat loss by signaling muscle tissue to uptake and metabolize free fatty acids. AdipoR1 and AdipoR2, two recently identified signaling receptors, mediate the actions of both adiponectin and gAcrp30.
Recombinant human gAcrp30, produced in E. coli, is a non-glycosylated polypeptide chain comprising 145 amino acids. With a molecular weight of 16.7 kDa, this protein is purified using proprietary chromatographic techniques.
The product is sterile-filtered and lyophilized from a solution containing 10mM sodium phosphate and 0.5mM DTT at a pH of 7.5.
For reconstitution, it is recommended to dissolve gAcrp30 at a concentration of 0.1 mg/ml in a solution of 10mM sodium phosphate and 0.5mM DTT, pH 7.5. This solution can be further diluted in other aqueous solutions as needed.
The purity of the protein is determined to be greater than 95% by SDS-PAGE analysis.
The biological activity of gAcrp30 is assessed based on its ability to suppress the proliferation of mouse M1 cells. The expected ED50 for this inhibitory effect is 669 ng/ml, which translates to a specific activity of 1.5 x 103 units/mg.
Product Science Overview
Adiponectin consists of a 60 amino acid N-terminal collagenous region and a 137 amino acid C-terminal C1q-like globular domain . The globular domain can be cleaved by leukocyte-derived elastase to form globular adiponectin . This globular form is biologically active and has been shown to have various metabolic effects.
Adiponectin plays a crucial role in regulating glucose levels and fatty acid breakdown. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity . The hormone is known for its anti-inflammatory properties in metabolic contexts, such as obesity and type 2 diabetes, but can have pro-inflammatory effects in non-metabolic disorders like rheumatoid arthritis and inflammatory bowel disease .
Recombinant human globular adiponectin is produced using various expression systems, including E. coli . The recombinant protein is often used in research to study its effects on metabolism and inflammation. It is supplied as a highly purified protein, with purity levels exceeding 90% as determined by SDS-PAGE .
Low levels of adiponectin are associated with obesity, insulin resistance, and type 2 diabetes . Increasing adiponectin levels or enhancing its receptor sensitivity is considered a potential therapeutic strategy for treating these conditions . Adiponectin receptors, AdipoR1 and AdipoR2, mediate the hormone’s effects on glucose uptake and fatty acid oxidation .
