Acrp30 Mouse, His

Adiponectin Mouse Recombinant, His Tag
Cat. No.
BT7382
Source
Escherichia Coli.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
Purity
Acrp30 Mouse purity is greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The Adiponectin Mouse is created as a recombinant protein with a 21 a.a N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is a 27.2kDa protein containing 251 amino acid residues of the Acrp30 Mouse, 18-247 amino acids.

Product Specs

Introduction
Adiponectin, primarily produced by adipocytes, is a secreted protein found in plasma. Its production increases during adipocyte differentiation, and insulin stimulates its secretion. Mouse adiponectin exhibits an 83% amino acid similarity to its human counterpart. Functionally, adiponectin plays a crucial role in energy regulation and is linked to obesity. Obese individuals often have reduced adiponectin levels, which is associated with insulin resistance and elevated insulin levels in the blood.
Description
The recombinant Adiponectin Mouse protein is engineered with a 21 amino acid His Tag fused to its N-terminal. Produced in E. coli, this His-Tagged Adiponectin Fusion Protein has a molecular weight of 27.2kDa. It encompasses amino acids 18-247 of the Acrp30 Mouse protein, along with the added His Tag, totaling 251 amino acid residues.
Formulation
Acrp30 Mouse is provided as a sterile, filtered solution at a concentration of 1mg/ml. The formulation buffer consists of 20mM Tris-HCl (pH 8.0), 1mM DTT (dithiothreitol), and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), Acrp30 Mouse should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of Acrp30 Mouse is confirmed to be greater than 90% through SDS-PAGE analysis.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N.

Product Science Overview

Structure and Expression

Recombinant mouse adiponectin is typically produced in Escherichia coli and includes a His tag for purification purposes. The His tag is a sequence of histidine residues added to the protein to facilitate its purification using metal affinity chromatography . The recombinant form of mouse adiponectin is a 27.2 kDa protein containing 251 amino acid residues .

Biological Functions

Adiponectin is an important adipokine involved in the control of fat metabolism and insulin sensitivity. It stimulates AMPK phosphorylation and activation in the liver and skeletal muscle, enhancing glucose utilization and fatty acid combustion . Additionally, adiponectin antagonizes TNF-alpha by negatively regulating its expression in various tissues such as the liver and macrophages .

Clinical Significance

Adiponectin levels are inversely correlated with obesity and insulin resistance. Lower levels of adiponectin are associated with increased risk of metabolic disorders such as type 2 diabetes and cardiovascular diseases . The protein’s ability to enhance insulin sensitivity and reduce inflammation makes it a potential therapeutic target for treating these conditions.

Applications

Recombinant mouse adiponectin with a His tag is widely used in research to study its biological functions and potential therapeutic applications. It is used in various assays, including sELISA and SDS-PAGE, to investigate its role in metabolic processes and its interactions with other proteins .

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THE BIOTEK
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