ACRP30 Rat

Adiponectin Rat Recombinant
Cat. No.
BT7714
Source
HEK293 cell line.
Synonyms
Adipocyte C1q and collagen domain-containing protein, Adipocyte complement-related 30kDa protein, Adipose most abundant gene transcript 1 protein, gelatin-binding protein, Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Appearance
White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

ACRP30 Rat Recombinant produced in 293 cell line is a polypeptide chain containing 238 amino acids and having a total molecular mass of 25.7 kDa. ACRP30 contains a C-Terminal linker (3 AA residue) and a C-Terminal Flag- tag (8 AA residue).

Product Specs

Introduction
Adiponectin, also known as Acrp30, AdipoQ, and GBP-28, is a 244-amino acid protein encoded by the apM1 gene. This protein plays an active physiological role and is primarily produced by adipose cells (making it an adipokine). Adiponectin belongs to the soluble defense collagen superfamily and features a collagen-like domain, structurally similar to collagen VIII, collagen X, and the globular domain of complement factor C1q. APM-1 assembles into homotrimers, which act as the foundation for larger complexes found circulating in serum.
Description
Recombinant Rat ACRP30, produced in a 293 cell line, is a polypeptide chain comprising 238 amino acids with a molecular weight of 25.7 kDa. This ACRP30 variant includes a C-terminal linker (3 amino acid residues) and a C-terminal Flag-tag (8 amino acid residues).
Physical Appearance
White, lyophilized powder.
Formulation
The product is filtered through a 0.4 μm filter and lyophilized in a 20 mM Tris buffer (pH 7.5) containing 50 mM NaCl.
Solubility
To prepare a working solution, add deionized water to achieve a concentration of approximately 0.5 mg/ml. Allow the lyophilized pellet to dissolve completely. This product is not sterile. Prior to using in cell culture, filter the product through an appropriate sterile filter.
Stability
Lyophilized ACRP30 remains stable at room temperature for 3 weeks. However, for extended storage, it is recommended to store the product desiccated at a temperature below -18°C. After reconstitution, ACRP30 should be stored at 4°C for 2 to 7 days. For long-term storage, it is advisable to add a carrier protein (0.1% HSA or BSA) and store the solution below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Adipocyte C1q and collagen domain-containing protein, Adipocyte complement-related 30kDa protein, Adipose most abundant gene transcript 1 protein, gelatin-binding protein, Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source
HEK293 cell line.
Amino Acid Sequence
GITATEGPGA LVPPPKETCA GWMAGIPGYP GHNGIPGRDG RDGTPGEKGE KGDAGVLGPK GDPGDAGMTG AEGPRGFPGT PGRKGEPGEA AYMYHSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFHCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSMLLHLEV GDQVWLQVYG EGDNNGLYAD NVNDSTFTGF LLYHDTNAAA DYKDDDDK

Product Science Overview

Introduction

Adiponectin, also known as Acrp30, AdipoQ, GBP-28, and apM1, is a 244-amino acid protein primarily secreted by adipose tissue. It was identified almost simultaneously by four different research groups using various approaches . Adiponectin is the most abundant peptide secreted by adipocytes and plays a crucial role in regulating metabolic processes, including glucose regulation and fatty acid oxidation .

Structure and Isoforms

Adiponectin circulates in the blood in three isoforms: high-molecular weight (HMW), middle-molecular weight (MMW), and low-molecular weight (LMW) complexes . These isoforms are produced through multimerization, a process involving several endoplasmic reticulum-associated proteins such as ER oxidoreductase 1-α (Ero1-α), ER resident protein 44 (ERp44), disulfide-bond A oxidoreductase-like protein (DsbA-L), and glucose-regulated protein 94 (GPR94) . The HMW complexes are particularly significant as they have predominant actions in metabolic tissues .

Biological Functions

Adiponectin has several important biological functions:

  1. Insulin Sensitization: Adiponectin enhances insulin sensitivity, making it a critical factor in the management of insulin resistance and type 2 diabetes .
  2. Anti-Inflammatory Effects: It exhibits anti-inflammatory properties, which help in reducing the risk of cardiovascular diseases .
  3. Anti-Atherogenic Effects: Adiponectin helps in preventing the formation of atherosclerotic plaques, thereby reducing the risk of cardiovascular diseases .
Mechanism of Action

Adiponectin exerts its effects through two main receptors, AdipoR1 and AdipoR2 . These receptors are expressed in various tissues, including the liver, skeletal muscle, and vascular endothelial cells. Upon binding to its receptors, adiponectin activates several signaling pathways that lead to increased glucose uptake and fatty acid oxidation .

Therapeutic Potential

Given its beneficial effects on metabolism, adiponectin has been studied as a potential therapeutic target for treating obesity, insulin resistance, type 2 diabetes, and cardiovascular diseases . Studies have shown that adiponectin administration in humans and rodents can have insulin-sensitizing, anti-atherogenic, and anti-inflammatory effects . In certain settings, it has also been observed to decrease body weight .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Acrp30 Human, His
Adiponectin Human Recombinant, His tag
Cat. No.
BT6313
Source
Escherichia Coli.
Acrp30 Human, HMW
Adiponectin glycosylated Human Recombinant, HMW Rich
Cat. No.
BT6377
Source
HEK293.
Acrp30 Human, Trimeric
Adiponectin Human Recombinant, Trimeric form
Cat. No.
BT6556
Source
HEK293 (Human embryonic kidney cell line).
gAcrp30 Human, His
Globular Adiponectin Human Recombinant, His Tag
Cat. No.
BT7973
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.