CNTFR Rat

Ciliary Neurotrophic Factor (CNTF) is a polypeptide trophic factor that belongs to the alpha-helical cytokine superfamily. It was initially identified for its ability to promote the survival of chick ciliary ganglion neurons in culture . CNTF is synthesized by glial cells in both the central nervous system (CNS) and peripheral nervous system (PNS), and it is ubiquitously distributed in neurons and glia throughout the rodent brain .

CNTF exerts its effects through a high-affinity receptor complex that includes the ligand-binding α-subunit (CNTFRα) and two signal-transducing β-subunits (leukemia inhibitory factor receptor, LIFRβ, and gp130) . The receptor complex is crucial for mediating the biological activities of CNTF, which include neuronal survival, differentiation, and gene expression .

Recombinant rat CNTF protein is produced in E. coli and is highly pure, biologically active, and sterile . It supports the growth and survival of dorsal root ganglion (DRG), motor, and sympathetic neurons . The recombinant protein is used in various research applications, including the study of neuronal survival and differentiation, as well as the activation of signaling pathways such as STAT3 and ERK .

CNTF has been shown to support the survival of various cell populations within the CNS and PNS . It promotes proliferation and neuronal specification in hippocampal neurons and delays neural degeneration in genetic models of motor neuron disease . Additionally, CNTF is involved in the development of astrocytes and oligodendrocytes .

The CNTFR gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of the tripartite receptor for CNTF . Binding of CNTF to CNTFR recruits the transmembrane components of the receptor, gp130 and LIFR, facilitating signal transduction . Single nucleotide polymorphisms in the CNTFR gene may be associated with variations in muscle strength and early onset of eating disorders .