CNTF Rat, His
Ciliary Neurotrophic Factor Rat Recombinant, His Tag
Cat. No.
BT7964
Source
Escherichia Coli.
Synonyms
Ciliary
neurotrophic factor, CNTF, Cntf.
Appearance
Sterile
filtered colorless solution.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CNTF Rat
Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain
containing 223 amino acids (1-200a.a) and having a molecular mass of 25.2kDa.
CNTF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CNTF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone primarily affecting the nervous system. It promotes neurotransmitter synthesis and neurite outgrowth in specific neuronal populations. CNTF acts as a potent survival factor for neurons and oligodendrocytes, potentially mitigating tissue damage during inflammation. Mutations in the CNTF gene can lead to aberrant splicing and CNTF deficiency, although this condition doesn't directly cause neurological diseases. Interestingly, the CNTF gene can be transcribed independently or co-transcribed with the upstream ZFP91 gene. Co-transcription produces a transcript encoding the full zinc finger protein but lacks a complete CNTF coding region. CNTF demonstrates neuroprotective properties by preventing motor axon degeneration following injury.
Description
Recombinant CNTF Rat, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 223 amino acids (1-200a.a) with a molecular mass of 25.2kDa. It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution free from particulate matter.
Formulation
The CNTF protein solution is provided at a concentration of 0.5mg/ml and is formulated in a buffer containing phosphate buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Purity
The purity of the CNTF protein is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Ciliary
neurotrophic factor, CNTF, Cntf.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN
INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS
AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS
ALESHYGAKD KQM.
Product Science Overview
Introduction
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone and neurotrophic factor primarily involved in the nervous system. It promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations, including astrocytes . The recombinant version of CNTF, particularly the rat recombinant with a His tag, is widely used in research to study its effects and potential therapeutic applications.
Structure and Function
CNTF is a member of the cytokine family and is encoded by the CNTF gene. The protein is a potent survival factor for neurons and oligodendrocytes, which are crucial for the maintenance and repair of the nervous system . The His tag, a sequence of histidine residues, is added to the recombinant protein to facilitate its purification and detection.
Biological Activities
CNTF has several biological activities, including:
- Neuroprotection: CNTF prevents the degeneration of motor axons after axotomy (nerve injury) and supports the survival of various neuronal cell types .
- Neurogenesis: It promotes the growth and differentiation of neurons, aiding in the development and repair of the nervous system .
- Anti-inflammatory Effects: CNTF may reduce tissue destruction during inflammatory attacks, making it a potential therapeutic agent for neuroinflammatory diseases .
Applications in Research
The rat recombinant CNTF with a His tag is used in various research applications, including:
- Neurodegenerative Disease Models: Studying the effects of CNTF in models of diseases like amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS).
- Neuronal Development Studies: Investigating the role of CNTF in the growth and differentiation of neurons.
- Drug Development: Exploring CNTF as a potential therapeutic agent for neurodegenerative and neuroinflammatory diseases.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
