CNTF Human, His

Ciliary Neurotrophic Factor Human Recombinant, His Tag
Cat. No.
BT7583
Source
Escherichia Coli.
Synonyms
HCNTF, CNTF, Ciliary Neurotrophic Factor.
Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Ciliary Neurotrophic Factor Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain (aa 1-200) containing a total of 220 amino acids and having a molecular mass of 25kDa. The CNTF protein is fused to a 20 aa His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ciliary neurotrophic factor (CNTF) is a polypeptide hormone primarily active in the nervous system. It promotes the synthesis of neurotransmitters and the growth of neurites in specific neuron populations. CNTF is crucial for the survival of neurons and oligodendrocytes, potentially mitigating tissue damage during inflammatory responses. Mutations in the CNTF gene can disrupt its splicing pattern, leading to ciliary neurotrophic factor deficiency, though this condition is not directly linked to neurological diseases. The CNTF gene exhibits complex transcription, producing a main monocistronic transcript and engaging in co-transcription with the ZFP91 gene upstream. This co-transcription generates a transcript containing the entire coding sequence for the zinc finger protein but lacking a complete coding sequence for CNTF. Notably, CNTF acts as a survival factor for various neuronal cell types and may prevent motor axon degeneration after injury.
Description
Recombinant Human Ciliary Neurotrophic Factor, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 1-200. With a molecular weight of 25 kDa, it includes a 20 amino acid His tag attached to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CNTF protein solution is provided at a concentration of 1 mg/ml and is formulated in 20mM Tris-HCl buffer at pH 8 with 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. To maintain protein integrity, avoid repeated freezing and thawing cycles.
Purity
SDS-PAGE analysis indicates a purity exceeding 95%.
Synonyms
HCNTF, CNTF, Ciliary Neurotrophic Factor.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSDLTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM.

Product Science Overview

Introduction

Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone and neurotrophic factor primarily involved in the nervous system. It is encoded by the CNTF gene in humans and plays a crucial role in promoting neurotransmitter synthesis and neurite outgrowth in specific neuronal populations . The recombinant version of human CNTF, often tagged with a His (histidine) tag for purification purposes, is widely used in research and therapeutic applications.

Structure and Function

CNTF is a protein with a molecular weight of approximately 23 kDa . It exerts its effects by binding to a heterotrimeric receptor complex composed of the CNTF receptor alpha (CNTFRα), glycoprotein 130 (gp130), and leukemia inhibitory factor receptor beta (LIFRβ) . This binding initiates a cascade of intracellular signaling pathways that promote cell survival, differentiation, and growth.

Biological Significance

CNTF is a potent survival factor for various neuronal cell types, including motor neurons and oligodendrocytes . It has been shown to prevent the degeneration of motor axons following axotomy (nerve injury) and may reduce tissue destruction during inflammatory attacks . Additionally, CNTF is involved in the regulation of gene expression, axon regeneration, and the activation of astrocytes .

Recombinant CNTF (Human, His Tag)

Recombinant human CNTF (rhCNTF) is produced using recombinant DNA technology, where the CNTF gene is cloned and expressed in a suitable host system, such as Escherichia coli or mammalian cells . The His tag, a sequence of histidine residues, is added to the protein to facilitate purification using affinity chromatography. This tag allows for efficient isolation of the recombinant protein from the host cell lysate.

Applications

Recombinant CNTF has several applications in research and medicine:

  • Neuroprotection: CNTF is used in studies investigating neuroprotective strategies for neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) .
  • Cell Culture: CNTF is often used as a supplement in cell culture media to support the growth and differentiation of neuronal cells .
  • Therapeutic Potential: CNTF has been explored as a potential therapeutic agent for various neurological conditions due to its ability to promote neuronal survival and regeneration .

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