CNTF Human, His Active

Ciliary Neurotrophic Factor Human Recombinant, His Tag Active
Cat. No.
BT7633
Source
Escherichia Coli.
Synonyms
Ciliary neurotrophic factor, CNTF, HCNTF.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CNTF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-200 a.a) and having a molecular mass of 25kDa.
CNTF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone primarily impacting the nervous system. It enhances neurotransmitter synthesis and neurite outgrowth in specific neuron populations. Acting as a potent survival factor for neurons and oligodendrocytes, CNTF might mitigate tissue damage during inflammatory responses. The gene encoding CNTF can undergo aberrant splicing due to mutations, leading to ciliary neurotrophic factor deficiency. However, this condition is not directly linked to neurological diseases. Interestingly, the CNTF gene shares a locus with the ZFP91 gene, leading to co-transcription. This results in a transcript with a complete coding sequence for the zinc finger protein but an incomplete one for CNTF. CNTF demonstrates neuroprotective properties by promoting the survival of various neuronal cell types and potentially preventing motor axon degeneration after injury.
Description
Recombinant human CNTF, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 220 amino acids (with a sequence spanning from amino acid position 1 to 200) and possessing a molecular weight of 25 kDa. For purification purposes, a 20 amino acid His-tag is fused to the N-terminus of CNTF, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The CNTF protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.5), 1 mM DTT, 30% Glycerol, and 0.2M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the CNTF vial should be stored at 4°C. For extended storage, it is recommended to store the protein frozen at -20°C. To ensure long-term stability, consider adding a carrier protein like HSA or BSA (0.1% concentration). It is important to minimize freeze-thaw cycles to maintain protein integrity.
Purity
The purity of CNTF is determined by SDS-PAGE analysis and is guaranteed to be greater than 95%.
Biological Activity
The biological activity of CNTF is assessed through a cell proliferation assay utilizing TF-1 human erythroleukemic cells. The ED50 for this effect, representing the concentration at which 50% of the maximal response is observed, is less than or equal to 1 ug/ml.
Synonyms
Ciliary neurotrophic factor, CNTF, HCNTF.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM.

Product Science Overview

Introduction

Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone and neurotrophic factor that plays a crucial role in the nervous system. It is encoded by the CNTF gene located on chromosome 11 in humans . CNTF is known for its ability to promote neurotransmitter synthesis and neurite outgrowth in certain neuronal populations, making it a potent survival factor for neurons and oligodendrocytes .

Structure and Function

CNTF is a protein that consists of 200 amino acids and has a molecular weight of approximately 22.9 kDa . The protein is primarily found in the cytoplasm and extracellular region, where it binds to the ciliary neurotrophic factor receptor (CNTFR) and activates various signaling pathways . These pathways are involved in the regulation of neuronal survival, differentiation, and growth .

The recombinant version of human CNTF, often referred to as rhCNTF, is produced using recombinant DNA technology. This version includes a His tag, which is a sequence of histidine residues added to the protein to facilitate purification and detection . The His tag does not interfere with the biological activity of CNTF and allows for efficient production of the protein in laboratory settings .

Biological Activities

CNTF has several important biological activities, including:

  • Neuronal Survival: CNTF is a potent survival factor for various neuronal cell types, including motor neurons and sensory neurons . It prevents the degeneration of motor axons after axotomy (cutting of an axon) and supports the survival of neurons under stress conditions .
  • Neurotransmitter Synthesis: CNTF promotes the synthesis of neurotransmitters, which are essential for communication between neurons .
  • Neurite Outgrowth: CNTF stimulates the outgrowth of neurites, which are projections from the cell body of a neuron that connect with other neurons or target tissues .
  • Inflammation Reduction: CNTF may play a role in reducing tissue destruction during inflammatory attacks, making it relevant in conditions such as multiple sclerosis .
Clinical Applications

Due to its neurotrophic properties, CNTF has been investigated for its potential therapeutic applications in various neurological disorders. Some of the conditions where CNTF has shown promise include:

  • Amyotrophic Lateral Sclerosis (ALS): CNTF has been studied for its potential to slow the progression of ALS, a neurodegenerative disease that affects motor neurons .
  • Retinal Degeneration: CNTF has been explored as a treatment for retinal degenerative diseases, such as retinitis pigmentosa, where it may help preserve photoreceptor cells .
  • Peripheral Neuropathy: CNTF has been evaluated for its ability to promote nerve regeneration and reduce symptoms in peripheral neuropathy .

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