CDC37 Human, His

Cell Division Cycle 37 Human Recombinant, His Tag
Cat. No.
BT6153
Source
E. coli
Synonyms
Cell Division Cycle 37 homolog, P50CDC37, CDC37, Hsp90 chaperone protein kinase-targeting subunit, CDC37 cell division cycle 37 homolog, hsp90 co-chaperone Cdc37, CDC37A.
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Usage
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Description

CDC37, His Protein is a 45.71 kDa protein containing 388 amino acids fused to a 10 aa
N-Terminal His-tag.

Product Specs

Introduction
CDC37, also known as Cell Division Cycle 37, is a protein highly homologous to Cdc37, a cell cycle control protein found in Saccharomyces cerevisiae (baker's yeast). This protein functions as a molecular chaperone and plays a crucial role in cellular signal transduction. CDC37 forms a complex with Hsp90 (Heat Shock Protein 90) and various protein kinases, including CDK4, CDK6, SRC, RAF-1, MOK, and eIF2 alpha kinases. Its primary function is to guide Hsp90 to its target kinases.
Description
CDC37, His Protein is a 45.71 kDa protein consisting of 388 amino acids. It also includes a 10 amino acid N-Terminal His-tag.
Formulation
The CDC37, His Human protein solution was filtered through a 0.4 micrometer filter and then lyophilized (freeze-dried) to achieve a concentration of 0.5mg/ml. It is supplied in a buffer solution containing 20mM TRIS, 50mM NaCl, with a pH of 7.5.
Solubility
To create a working stock solution of 0.5 mg/ml, add deionized water to the lyophilized pellet and allow it to dissolve completely. Please note that this product is not sterile. It is essential to filter the solution through an appropriate sterile filter before using it in cell culture.
Stability
Store the lyophilized CDC37 His human protein at -20°C. After reconstituting the protein, it is recommended to aliquot it into smaller portions to prevent repeated cycles of freezing and thawing. Reconstituted CDC37 remains stable at 4°C for a limited period and shows no significant change after two weeks of storage at this temperature.
Synonyms
Cell Division Cycle 37 homolog, P50CDC37, CDC37, Hsp90 chaperone protein kinase-targeting subunit, CDC37 cell division cycle 37 homolog, hsp90 co-chaperone Cdc37, CDC37A.
Source
E. coli
Amino Acid Sequence
MKHHHHHHAS MVDYSVWDHI EVSDDEDETH PNIDTASLFR WRHQARVERM EQFQKEKEEL DRGCRECKRK VAECQRKLKE LEVAEGGKAE LERLQAEAQQ LRKEERSWEQ KLEEMRKKEK SMPWNVDTLS KDGFSKSMVN TKPEKTEEDS EEVREQKHKT FVEKYEKQIK HFGMLRRWDD SQKYLSDNVH LVCEETANYL VIWCIDLEVE EKCALMEQVA HQTIVMQFIL ELAKSLKVDP RACFRQFFTK IKTADRQYME GFNDELEAFK ERVRGRAKLR IEKAMKEYEE EERKKRLGPG GLDPVEVYES LPEELQKCFD VKDVQMLQDA ISKMDPTDAK YHMQRCIDSG LWVPNSKASE AKEGEEAGPG DPLLEAVPKT GDEKDVSV

Product Science Overview

Structure and Function

CDC37 is highly similar to the cell division cycle control protein found in Saccharomyces cerevisiae (yeast). The human CDC37 protein is composed of 388 amino acids and has a molecular weight of approximately 45.71 kDa . It is often tagged with a His (histidine) tag to facilitate purification and detection in laboratory experiments.

CDC37 forms a complex with Heat Shock Protein 90 (Hsp90) and several protein kinases, including CDK4, CDK6, SRC, RAF-1, MOK, and eIF2 alpha kinases . This complex is vital for directing Hsp90 to its target kinases, thereby stabilizing and promoting their activity . CDC37 also inhibits the ATPase activity of Hsp90, which is essential for its chaperone function .

Role in Disease

Mutations or dysregulation of CDC37 have been associated with various diseases, including Fanconi Anemia and Parkinson’s Disease . Its role in stabilizing protein kinases makes it a critical player in cell cycle regulation and signal transduction pathways, which are often disrupted in cancer and other diseases.

Applications in Research

Recombinant CDC37 tagged with a His tag is widely used in research to study its function and interactions with other proteins. The His tag allows for easy purification using nickel affinity chromatography, making it a valuable tool for biochemical and structural studies . Researchers can use recombinant CDC37 to investigate its role in various signaling pathways and its potential as a therapeutic target.

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