CDC34 Human, His
Description
CDC34 Human Recombinant protein fused to a 20 amino acid N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 256 amino acids (1-236 a.a.) and having a molecular mass of 28.9 kDa. CDC34 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE
YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES.
Product Science Overview
The human recombinant CDC34 protein is typically produced in Escherichia coli (E. coli) and is fused to a 20 amino acid N-terminal His-Tag. This recombinant protein is a single, non-glycosylated polypeptide chain containing 256 amino acids, with a molecular mass of approximately 28.9 kDa . The His-Tag facilitates purification through affinity chromatography techniques.
CDC34 is essential for the ubiquitin-mediated degradation of cell cycle G1 regulators and the initiation of DNA replication. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to target proteins. This process is crucial for the regulation of various cellular processes, including cell division, signal transduction, and development .
CDC34 works in association with different E3 complexes, such as the SCF (Skp, Cullin, F-box containing complex), to target substrates for ubiquitination and subsequent degradation. Some of the well-characterized substrates of CDC34 include IκB, B-Myb, Wee1, MyoD, ICERIIγ, ATF5, p27Xic1, and p27Kip1 . Additionally, substrates like β-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative targets of CDC34 due to their requirement for SCF-mediated proteolysis .
The recombinant CDC34 protein is widely used in in vitro ubiquitination reactions to study the ubiquitin-proteasome pathway and its role in various cellular processes. Its ability to self-associate through a domain in the C-terminus and its phosphorylation and ubiquitinylation in vivo make it a valuable tool for research .
The CDC34 protein solution is typically formulated with 20mM Tris-HCl (pH 8), 10% glycerol, 50mM NaCl, and 1mM DTT. It should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended to avoid multiple freeze-thaw cycles .
