CDC26 Human

CDC26 is a single, non-glycosylated polypeptide chain consisting of 105 amino acids, with a molecular mass of approximately 11.9 kDa . The recombinant form of CDC26 is often produced in Escherichia coli (E. coli) and is fused to a 20 amino acid His-tag at the N-terminus to facilitate purification .

The primary function of CDC26 within the APC/C complex is to enhance the ubiquitination of cell-cycle regulators, thereby controlling their degradation. This process is vital for the orderly progression of the cell cycle, particularly during the transition from metaphase to anaphase . The APC/C complex mainly mediates the formation of ‘Lys-11’-linked polyubiquitin chains and, to a lesser extent, ‘Lys-48’- and ‘Lys-63’-linked polyubiquitin chains .

CDC26 is highly conserved across species, indicating its fundamental role in cell cycle regulation. The protein encoded by the CDC26 gene is similar to the Saccharomyces cerevisiae Cdc26, which is also a component of the APC . The APC/C complex, including CDC26, is responsible for the cell cycle-regulated proteolysis of various proteins, ensuring that cells progress through the cell cycle in a controlled manner .

Recombinant CDC26 is used extensively in laboratory research to study cell cycle regulation and the mechanisms of ubiquitination. It is available in various quantities and is typically supplied as a sterile, filtered, colorless solution containing Tris-HCl buffer, glycerol, and NaCl . The protein is stable when stored at 4°C for short periods or at -20°C for long-term storage, with the addition of a carrier protein to prevent degradation .