CDC25A Human
Cell Division Cycle 25A Human Recombinant
Cat. No.
BT5762
Source
Escherichia Coli.
Synonyms
M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A, CDC25A2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CDC25A Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 560 amino acids (1-524 a.a.) and having a molecular mass of 63.2kDa. The CDC25A is purified by proprietary chromatographic techniques.
Product Specs
Introduction
M-phase inducer phosphatase 1 (CDC25A), a member of the CDC25 phosphatase family, plays a crucial role in cell cycle progression from the G1 to the S phase. It activates cyclin-dependent kinase CDC2 by removing two phosphate groups, thereby promoting cell cycle advancement. In response to DNA damage, CDC25A undergoes degradation, preventing cells with chromosomal abnormalities from proceeding through cell division. While recognized as an oncogene, the precise role of CDC25A in oncogenesis remains to be fully elucidated.
Description
Recombinant human CDC25A, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 63.2 kDa. It consists of 560 amino acids (1-524 a.a.) and includes a 36 amino acid His tag at the N-terminus. Purification of CDC25A is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CDC25A solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 5mM DTT, 20% glycerol, 0.2M NaCl, and 1mM EDTA.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of CDC25A is determined by SDS-PAGE analysis to be greater than 80.0%.
Synonyms
M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A, CDC25A2.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQG LGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENE AFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETP SCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKG TIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLK KPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTK SRTWAGEKSK REMYSRLKKL.
Product Science Overview
Gene and Protein Information
Function and Mechanism
CDC25A is essential for the progression from the G1 phase to the S phase of the cell cycle. It achieves this by removing inhibitory phosphate groups from CDK2-cyclin E and CDK2-cyclin A complexes . Additionally, CDC25A has a role during the G2-M transition by activating CDK1-cyclin B complexes, which are thought to initiate chromosome condensation .
Regulation and Degradation
CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division . This degradation is part of the DNA damage checkpoint mechanism and involves the ubiquitin-proteasome pathway . The rapid degradation of CDC25A in response to genotoxic stress helps maintain genome integrity by halting cell cycle progression and allowing time for DNA repair .
Clinical Significance
Subcellular Localization
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
