CCNH Human

Cyclin-H forms a trimeric complex with CDK7 and MAT1, known as the CDK-activating kinase (CAK) complex . This complex is essential for the activation of CDKs, which are critical for cell cycle progression. Cyclin-H is also a component of the transcription factor TFIIH, which is involved in transcription initiation and DNA repair .

Recombinant human Cyclin-H is typically produced using various expression systems, such as E. coli or insect cells. The recombinant protein is often tagged with a His-tag or GST-tag to facilitate purification . For example, one method involves expressing full-length human Cyclin-H in insect cells using an N-terminal GST tag . Another method involves expressing Cyclin-H with a His-tag in E. coli .

Recombinant Cyclin-H is used in various research applications, including studies on cell cycle regulation, transcription, and DNA repair. It is also used to investigate the mechanisms of CDK activation and the role of the CAK complex in cell cycle control .

Recombinant Cyclin-H is typically stored at -70°C to maintain its stability and activity. It is supplied in a buffer containing Tris-HCl, NaCl, glycerol, and other stabilizing agents . Proper storage and handling are essential to avoid repeated freeze-thaw cycles, which can degrade the protein .