CCNH Antibody

The CAK complex regulates cell cycle progression by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes . The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH), which can affect its substrate specificity . When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II, linking cell cycle control, transcriptional regulation, and DNA repair .

The Mouse Anti Human Cyclin-H Antibody is a polyclonal antibody produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of cyclin H . These antibodies are purified by protein A and peptide affinity chromatography . The antibody detects endogenous levels of cyclin H and does not cross-react with other family members at physiological levels .

This antibody is validated for use in various applications, including:

• Western Blotting (WB): Used to detect cyclin H in cell extracts.
• Immunoprecipitation (IP): Used to isolate cyclin H from cell lysates .

The antibody is supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, and 50% glycerol. It should be stored at –20°C and should not be aliquoted .